Title: Species difference in 1,3-dinitrobenzene testicular toxicity: in vitro correlation with glutathione status.

Authors: Jacobson, C F; Miller, M G

Published In Reprod Toxicol, (1998 Jan-Feb)

Abstract: It has been previously shown that 1,3-dinitrobenzene (DNB) causes testicular damage to the rat but not the hamster. The present study of DNB's mechanism of toxic action has exploited this species difference in susceptibility. Seminiferous tubules were isolated from Golden Syrian hamsters and incubated with 100 microM DNB or vehicle for 22 h. (A similar study with rat tubules has been published.) Formation of DNB metabolites were monitored over time; hamster tubules had a greater capacity than rat tubules for reductively metabolizing (activating) DNB. However, hamster tubules did not show the marked DNB-induced ATP depletion seen in rat tubules. Levels of mitochondrial glutathione and activities of enzymes that protect against oxidative stress were measured in both rat and hamster tubules. The observed differences in the capacity for detoxification of oxidants may underlie the difference in susceptibility to DNB-induced testicular toxicity between these species.

PubMed ID: 9431572

MeSH Terms: Adenosine Triphosphate/metabolism; Animals; Antioxidants/metabolism; Cricetinae; Dinitrobenzenes/metabolism; Dinitrobenzenes/pharmacokinetics; Dinitrobenzenes/toxicity*; Glutathione/metabolism*; Inactivation, Metabolic; Male; Mesocricetus; Mitochondria/drug effects; Mitochondria/enzymology; Mitochondria/metabolism; Oxidative Stress; Proteins/metabolism; Rats; Seminiferous Tubules/drug effects; Seminiferous Tubules/enzymology; Seminiferous Tubules/metabolism; Species Specificity; Testicular Diseases/chemically induced*; Testicular Diseases/metabolism*