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Title: Tissue specificity of spontaneous point mutations in lambda supF transgenic mice.

Authors: Leach, E G; Narayanan, L; Havre, P A; Gunther, E J; Yeasky, T M; Glazer, P M

Published In Environ Mol Mutagen, (1996)

Abstract: Transgenic mice carrying multiple copies of a recoverable lambda phage shuttle vector carrying the supF mutation reporter gene (lambda supF) were constructed for the purpose of studying mutagenesis in a whole animal. Spontaneous mutations in rescued supF target genes from mouse liver and skin were analyzed. The mutation frequency was similar in both tissues (in the range of 2 x 10(-5)), but the spectrum of point mutations was distinct, with transitions common in the skin and transversions more prominent in the liver (P = 0.01). These results may help to elucidate pathways of endogenous mutagenesis in vivo, and they illustrate potentially important tissue-specific differences in genetic instability.

PubMed ID: 8991078 Exiting the NIEHS site

MeSH Terms: Animals; Bacteriophage lambda/genetics*; Base Sequence; Female; Genes, Suppressor; Liver/physiology; Mice; Mice, Transgenic/genetics*; Molecular Sequence Data; Mutation; Organ Specificity*; Point Mutation*; RNA, Transfer/genetics*; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Sequence Analysis, DNA; Skin Physiology

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