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Publication Detail

Title: Physiological and cellular responses of the 2,4-D degrading bacterium, Burkholderia cepacia YK-2, to the phenoxyherbicides 2,4-D and 2,4,5-T.

Authors: Cho, Yun-Seok; Kahng, Hyung-Yeel; Kim, Chi-Kyung; Kukor, Jerome J; Oh, Kye-Heon

Published In Curr Microbiol, (2002 Dec)

Abstract: Our previous research has demonstrated that novel 43-kDa DnaK and 41-kDa GroEL proteins are synthesized in Burkholderia sp. YK-2 in response to sublethal concentrations of 2,4-D stress [Cho et al. (2000) Curr Microbiol 41:33-38]. In this study, we have extended this work to examine the cellular responses of strain YK-2 to stresses induced in response to the phenoxyherbicides 2,4-D or 2,4,5-T. Strain YK-2 exhibited a more sensitive response to 2,4,5-T stress than to 2,4-D stress, as shown in physiological and morphological changes, suggesting a greater cytotoxic effect of 2,4,5-T. SEM analyses revealed the presence of perforations and irregular rod forms with wrinkled surfaces for cells treated with either herbicide. These irregularities were found more frequently for 2,4,5-T-treated cells than for 2,4-D-treated cells. Analysis of cellular fatty acids showed similar effects in the shifts of total cellular fatty acid composition in response to 2,4-D and 2,4,5-T. Strain YK-2 could degrade 2.25 m M 2,4-D completely during 28 h of incubation with transient production of 2,4-dichlorophenol as a metabolite; however, 2,4,5-T was not catabolized at any of the concentrations tested. BIOLOG and 16S rDNA analyses revealed that strain YK-2 was 98% similar to the Burkholderia cepacia species cluster; therefore, we have designated this strain as B. cepacia YK-2.

PubMed ID: 12402082 Exiting the NIEHS site

MeSH Terms: 2,4,5-Trichlorophenoxyacetic Acid/metabolism; 2,4,5-Trichlorophenoxyacetic Acid/toxicity*; 2,4-Dichlorophenoxyacetic Acid/metabolism; 2,4-Dichlorophenoxyacetic Acid/toxicity*; Biodegradation; Burkholderia cepacia/drug effects; Burkholderia cepacia/genetics; Burkholderia cepacia/physiology*; Burkholderia cepacia/ultrastructure*; DNA, Ribosomal/analysis; Fatty Acids/analysis; Heat-Shock Response; Herbicides/metabolism; Herbicides/pharmacology; Herbicides/toxicity*; Microscopy, Electron, Scanning; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S/genetics; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Sequence Analysis, DNA

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