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National Institute of Environmental Health Sciences

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Publication Detail

Title: Receptor-mediated endocytosis of soluble and membrane-tethered Sonic hedgehog by Patched-1.

Authors: Incardona, J P; Lee, J H; Robertson, C P; Enga, K; Kapur, R P; Roelink, H

Published In Proc Natl Acad Sci U S A, (2000 Oct 24)

Abstract: Patched (Ptc) is the ligand-binding component of the Hedgehog (Hh) receptor complex. In the Drosophila embryo, Ptc and Hh colocalize in vesicular punctate structures. However, receptor-mediated endocytosis of Hh proteins has not been demonstrated. By using chick neural plate explants, we show that Sonic hedgehog (Shh)-responsive neural precursor cells internalize recombinant and endogenous Shh and provide direct evidence for a gradient of endogenous Shh in the ventral neural tube. Shh internalization is blocked by a monoclonal antibody whose epitope overlaps the Ptc-binding site of Shh. These findings suggest that Shh internalization is mediated by Ptc-1 and may be linked to signaling. Concordantly, transfection of mammalian cell lines with a Ptc-1 cDNA confers the ability to internalize multiple forms of Shh, including transmembrane-anchored Shh, by a dynamin-dependent process.

PubMed ID: 11027307 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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