Title: Exploring bioanalytical applications of assisted protein reassembly.

Authors: Deo, Sapna K

Published In Anal Bioanal Chem, (2004 Jun)

Abstract: Reassembly of protein from its peptide fragments is a technique that can have many applications in the bioanalytical field. Typically, a reporter protein fragmented into its two peptides is employed as a label in this study. This fragments of peptide can reassemble yielding an active functional reporter. This reassembly of the protein can be assisted by non-covalently interacting peptides or proteins, which are attached to the fragmented reporter. This technique has been employed in several applications including study of protein-protein interactions, antibody screening, immunoassays, and high-throughput screening. This review focuses on different reporters employed in the study of reassembly of proteins and applications of this strategy in bioanalysis.

PubMed ID: 15127176

MeSH Terms: No MeSH terms associated with this publication