Title: The effects of polybrominated biphenyls and perchlorinated terphenyls on in vitro fertilization in the mouse.

Authors: Kholkute, S D; Rodriguez, J; Dukelow, W R

Published In Arch Environ Contam Toxicol, (1994 Feb)

Abstract: Polybrominated biphenyls (PBBs) and perchlorinated terphenyls (PCTs) are industrial chemicals that are long-lasting environmental contaminants. Although in vivo effects of PBBs on reproduction are documented, no information is available on the effects of these chemicals on sperm-egg interactions or fertilization. The present study was undertaken to determine the toxic potential of PBBs and PCTs on in vitro fertilization (IVF) in the mouse. 2-Bromobiphenyl, 4-bromobiphenyl, o-terphenyl, m-terphenyl, and p-terphenyl were added to the IVF medium at various concentrations. Oocytes collected from superovulated B6D2F1 mice were maintained in a medium containing the chemicals. Capacitated sperm were then added and the dishes cultured in a humidified atmosphere at 37 degrees C in 5% CO2 + 95% air. Oocytes were assessed for fertilization 20-24 h after insemination. polybrominated biphenyls and PCTs reduced the IVF rate at the higher dosages. Furthermore, an increased incidence of abnormal two-cell embryos and degenerative oocytes was observed at the 1 and 10 micrograms/ml concentrations of PBBs and PCTs. These results indicate that PBBs and PCTs adversely effect IVF and increase the incidence of abnormal embryos and oocyte degeneration in the mouse.

PubMed ID: 8311512

MeSH Terms: Animals; Cells, Cultured; Embryo, Mammalian/drug effects; Female; Fertilization in Vitro/drug effects*; Male; Mice; Polychlorinated Biphenyls/toxicity*; Terphenyl Compounds/toxicity*