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National Institute of Environmental Health Sciences

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Publication Detail

Title: Microencapsulation of single, multiple, and zona pellucida-free mouse preimplantation embryos in sodium alginate and their development in vitro.

Authors: Cosby, N C; Dukelow, W R

Published In J Reprod Fertil, (1990 Sep)

Abstract: Preimplantation embryos obtained from immature superovulated B6D2F1 female mice were microencapsulated in sodium alginate singly, in multiples of 2 or 3, or denuded of their zona pellucida. Encapsulated embryos developed in vitro at a rate similar to control embryos. Development of zona pellucida-free embryos was significantly less than that of intact embryos, but there was no difference between encapsulated and non-encapsulated zona pellucida-free embryos. Development of 2- and 4-cell embryos in sodium alginate was independent of cell stage. This report demonstrates the usefulness of a viable, biodegradable embedding material for the microencapsulation of manipulated preimplantation mammalian embryos.

PubMed ID: 2231540 Exiting the NIEHS site

MeSH Terms: Alginates/pharmacology*; Animals; Blastocyst/cytology; Blastocyst/physiology*; Cells, Cultured; Culture Media; Embryonic and Fetal Development*; Glucuronic Acid; Hexuronic Acids; Mice; Mice, Inbred Strains; Micromanipulation/methods; Zona Pellucida/physiology

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