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Publication Detail

Title: A new model of the blood--brain barrier: co-culture of neuronal, endothelial and glial cells under dynamic conditions.

Authors: Stanness, K A; Neumaier, J F; Sexton, T J; Grant, G A; Emmi, A; Maris, D O; Janigro, D

Published In Neuroreport, (1999 Dec 16)

Abstract: Developing in vitro blood-brain barrier (BBB) models that closely mimic the natural state is important for theoretical and practical applications, including drug development. We previously developed an in vitro BBB model based on co-culturing endothelial cells with glia in the presence of flow on hollow fiber tube culture substrates. We now report that this dynamic in vitro BBB (DIV-BBB) can be successfully used to co-culture differentiated serotonergic neurons in the presence of a BBB. These neurons demonstrated fluoxetine-sensitive serotonin (5HT) uptake and depolarization-induced release of [3H]5HT. Our results demonstrate that the DIV-BBB is a suitable model for culturing of neurons in a quasi-physiological microenvironment and in the presence of a high-resistance, stereoselective BBB.

PubMed ID: 10716199 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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