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Publication Detail

Title: Preliminary purification and characterization studies of a low molecular weight, high affinity cytosolic lead-binding protein in rat brain.

Authors: DuVal, G; Fowler, B A

Published In Biochem Biophys Res Commun, (1989 Feb 28)

Abstract: Carrier-free 203Pb has been used to label high affinity lead-binding proteins in rat brain cytosol to allow their initial characterization. The low molecular weight 203Pb-protein complex collected from a Sephadex G-75 column eluate has been further purified by Sephadex DEAE chromatography and then partially characterized. The protein has a molecular weight of 23,000 daltons as determined by SDS polyacrylamide gel electrophoresis and significant levels of glutamic acid (9.3%), aspartic acid (10.8%) and cysteine (9.4%). Western blot studies conducted using the polyclonal antibody to the renal lead-binding proteins showed a lack of reactivity, indicating that the brain protein is immunologically distinct from that found in the kidney.

PubMed ID: 2564273 Exiting the NIEHS site

MeSH Terms: No MeSH terms associated with this publication

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