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National Institute of Environmental Health Sciences

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Your Environment. Your Health.

Person Details: Jun Nakamura

Superfund Research Program

Jun Nakamura

University of North Carolina-Chapel Hill
Department of Environmental Sciences and Engineering
School of Public Health
CB# 7431, Rosenau Hall
Chapel Hill, North Carolina 27599-7431
Phone: 919-966-6140
Fax: 919-966-6123
Email: ynakamur@email.unc.edu

Projects

Patents

Publications

2017

  • De La Rosa VY, Asfaha J, Fasullo M, Loguinov AV, Li P, Moore LE, Rothman N, Nakamura J, Swenberg JA, Scelo G, Zhang L, Smith MT, Vulpe CD. 2017. Editor's highlight: High-throughput functional genomics identifies modulators of TCE metabolite genotoxicity and candidate susceptibility genes. Toxicol Sci 160:111-120. doi:10.1093/toxsci/kfx159 PMID:28973557 PMCID:PMC5837773
  • Nakamura J, Shimomoto T, Collins LB, Holley DW, Zhang Z, Barbee JM, Sharma V, Tian X, Kondo T, Uchida K, Yi X, Perkins DO, Willis MS, Gold A, Bultman SJ. 2017. Evidence that endogenous formaldehyde produces immunogenic and atherogenic adduct epitopes. Sci Rep 7:12. doi:10.1038/s41598-017-11289-8 PMID:28883613 PMCID:PMC5589919
  • Shimomoto T, Collins LB, Yi X, Holley DW, Zhang Z, Tian X, Uchida K, Wang C, Horkko S, Bultman SJ, Willis MS, Gold A, Nakamura J. 2017. A purified MAA-based ELISA is a useful tool for determining anti-MAA antibody titer with high sensitivity. PLoS One 12(2):e0172172. doi:10.1371/journal.pone.0172172 PMID:28222187 PMCID:PMC5319763
  • Tian Z, Gold A, Nakamura J, Zhang Z, Vila J, Singleton D, Collins LB, Aitken MD. 2017. Nontarget Analysis Reveals a Bacterial Metabolite of Pyrene Implicated in the Genotoxicity of Contaminated Soil after Bioremediation. Environ Sci Technol 51(12):7091-7100. doi:10.1021/acs.est.7b01172 PMID:28510420 PMCID:PMC6309544

2016

  • Adrion AC, Nakamura J, Shea D, Aitken MD. 2016. Screening nonionic surfactants for enhanced biodegradation of polycyclic aromatic hydrocarbons remaining in soil after conventional biological treatment. Environ Sci Technol 50(7):3838-3845. doi:10.1021/acs.est.5b05243 PMID:26919662 PMCID:PMC4973855
  • Adrion AC, Singleton D, Nakamura J, Shea D, Aitken MD. 2016. Improving polycyclic aromatic hydrocarbon biodegradation in contaminated soil through low-level surfactant addition after conventional bioremediation. Environ Eng Sci 33(9):659-670. doi:10.1089/ees.2016.0128 PMID:27678476 PMCID:PMC5031096
  • Gmeiner WH, Gearhart PJ, Pommier Y, Nakamura J. 2016. F10 cytotoxicity via topoisomerase I cleavage complex repair consistent with a unique mechanism for thymineless death. Future Oncol 12(19):2183-2188. doi:10.2217/fon-2016-0127 PMID:27333295
  • Hashimoto K, Sharma V, Sasanuma H, Tian X, Takata M, Takeda S, Swenberg JA, Nakamura J. 2016. Poor recognition of O6-isopropyl dG by MGMT triggers double strand break-mediated cell death and micronucleus induction in FANC-deficient cells. Oncotarget 7(37):59795-59808. doi:10.18632/oncotarget.10928 PMID:27486975
  • Sharma V, Collins LB, Chen T, Herr N, Takeda S, Sun W, Swenberg JA, Nakamura J. 2016. Oxidative stress at low levels can induce clustered DNA lesions leading to NHEJ mediated mutations. Oncotarget 7(18):25377-25390. doi:10.18632/oncotarget.8298 PMID:27015367 PMCID:PMC5041911
  • Swenberg JA, Lai Y, Yu R, Sharma V, Moeller BC, Hartwell HJ, MacDonald Gibson J, Nakamura J. 2016. The role of endogenous versus exogenous DNA damage in risk assessment. In: Thresholds of genotoxic carcinogens: from mechanisms to regulation. Elsevier, pp.83-102.
  • Tian X, Patel K, Ridpath JR, Chen Y, Zhou Y, Neo D, Clement JM, Takata M, Takeda S, Sale J, Wright FA, Swenberg JA, Nakamura J. 2016. Homologous recombination and translesion DNA synthesis play critical roles on tolerating DNA damage caused by trace levels of hexavalent chromium. PLoS One 11(12):e0167503. doi:10.1371/journal.pone.0167503 PMID:27907204 PMCID:PMC5132242

2015

  • Chibwe L, Geier MC, Nakamura J, Tanguay RL, Aitken MD, Simonich SL. 2015. Aerobic bioremediation of PAH contaminated soil results in increased genotoxicity and developmental toxicity. Environ Sci Technol 49(23):13889-13898. doi:10.1021/acs.est.5b00499 PMID:26200254 PMCID:PMC4666737
  • Hashimoto K, Takeda S, Swenberg JA, Nakamura J. 2015. Incorporation of metabolic activation potentiates cyclophosphamide-induced DNA damage response in isogenic DT40 mutant cells. Mutagenesis 30(6):821-828. doi:10.1093/mutage/gev042 PMID:26085549 PMCID:PMC4723676
  • Hirota K, Yoshikiyo K, Guilbaud G, Tsurimoto T, Murai J, Tsuda M, Phillips LG, Narita T, Nishihara K, Kobayashi K, Yamada K, Nakamura J, Pommier Y, Lehmann A, Sale JE, Takeda S. 2015. The POLD3 subunit of DNA polymerase δ can promote translesion synthesis independently of DNA polymerase ζ. Nucleic Acids Res 43(3):1671-1683. doi:10.1093/nar/gkv023 PMID:25628356 PMCID:PMC4330384

2014

2013

  • Hira A, Yabe H, Yoshida K, Okuno Y, Shiraishi Y, Chiba K, Tanaka H, Miyano S, Nakamura J, Kojima S, Ogawa S, Matsuo K, Takata M, Yabe M. 2013. Variant ALDH2 is associated with accelerated progression of bone marrow failure in Japanese Fanconi anemia patients. Blood 122(18):3206-3209. doi:10.1182/blood-2013-06-507962 PMID:24037726 PMCID:PMC3953058
  • Pachkowski BF, Nakamura J. 2013. A neutral glyoxal gel electrophoresis method for the detection and semi-quantitation of DNA single-strand breaks. Methods Mol Biol 1054:133-143. doi:10.1007/978-1-62703-565-1_8 PMID:23913289

2012

  • Hu J, Nakamura J, Richardson SD, Aitken MD. 2012. Evaluating the effects of bioremediation on genotoxicity of polycyclic aromatic hydrocarbon-contaminated soil using genetically engineered, higher eukaryotic cell lines. Environ Sci Technol 46(8):4607-4613. doi:10.1021/es300020e PMID:22443351 PMCID:PMC3348858
  • Lu K, Craft S, Nakamura J, Moeller BC, Swenberg JA. 2012. Use of LC-MS/MS and stable isotopes to differentiate hydroxymethyl and methyl DNA adducts from formaldehyde and nitrosodimethylamine. Chem Res Toxicol 25(3):664-675. doi:10.1021/tx200426b PMID:22148432 PMCID:PMC3307879
  • Luke AM, Nakamura J. 2012. O-hydroxylamine-coupled alkaline gel electrophoresis assay for the detection and measurement of DNA single-strand breaks. Methods Mol Biol 920:307-313. doi:10.1007/978-1-61779-998-3_21 PMID:22941612 PMCID:PMC3664990
  • Nakamura J, Gul H, Tian X, Bultman SJ, Swenberg JA. 2012. Detection of PIGO-deficient cells using proaerolysin: a valuable tool to investigate mechanisms of mutagenesis in the DT40 cell system. PLoS One 7(3):e33563. doi:10.1371/journal.pone.0033563 PMID:22428069 PMCID:PMC3299801

2011

  • Luke AM, Chastain PD, Pachkowski BF, Afonin V, Takeda S, Kaufman DG, Swenberg JA, Nakamura J. 2011. Accumulation of true single strand breaks and AP sites in base excision repair deficient cells. Mutat Res 694:65-71. doi:10.1016/j.mrfmmm.2010.08.008 PMID:20851134 PMCID:PMC2992575
  • Ridpath JR, Takeda S, Swenberg JA, Nakamura J. 2011. Convenient, multi-well plate-based DNA damage response analysis using DT40 mutants is applicable to a high-throughput genotoxicity assay with characterization of modes of action. Environ Mol Mutagen 52(2):153-160. doi:10.1002/em.20595 PMID:20839229 PMCID:PMC3280086
  • Swenberg JA, Lu K, Moeller BC, Gao L, Upton PB, Nakamura J, Starr TB. 2011. Endogenous versus exogenous DNA adducts: their role in carcinogenesis, epidemiology, and risk assessment. Toxicol Sci 120(suppl1):S130-S145. doi:10.1093/toxsci/kfq371 PMID:21163908 PMCID:PMC3043087

2010

  • Asagoshi K, Tano K, Chastain PD, Adachi N, Sonoda E, Kikuchi K, Koyama H, Nagata K, Kaufman DG, Takeda S, Wilson SH, Watanabe M, Swenberg JA, Nakamura J. 2010. FEN1 functions in long patch base excision repair under conditions of oxidative stress in vertebrate cells. Mol Cancer Res 8(2):204-215. doi:10.1158/1541-7786.MCR-09-0253 PMID:20145043 PMCID:PMC2824787
  • Chastain PD, Nakamura J, Rao S, Chu H, Ibrahim J, Swenberg JA, Kaufman DG. 2010. Abasic sites preferentially form at regions undergoing DNA replication. FASEB J 24(10):3674-80. doi:10.1096/fj.09-145276 PMID:20511393 PMCID:PMC2996904

2009

2008

  • Brusick D, Small MS, Cavalieri EL, Chakravarti D, Ding X, Longfellow DG, Nakamura J, Rogan EC, Swenberg JA. 2008. Possible genotoxic modes of action for naphthalene. Regul Toxicol Pharmacol 51(2Suppl):S43-S50. doi:10.1016/j.yrtph.2007.12.002 PMID:18194829

2007

  • Ridpath JR, Nakamura J, Tano K, Luke AM, Sonoda E, Arakawa H, Buerstedde J, Gillespie DA, Sale JE, Yamazoe M, Bishop DK, Takata M, Takeda S, Watanabe M, Swenberg JA. 2007. Cells deficient in the FANC/BRCA pathway are hypersensitive to plasma levels of formaldehyde. Cancer Res 67(23):11117-11122. doi:10.1158/0008-5472.CAN-07-3028 PMID:18056434
  • Tano K, Nakamura J, Asagoshi K, Arakawa H, Sonoda E, Braithwaite EK, Prasad R, Buerstedde J, Takeda S, Watanabe M, Wilson SH. 2007. Interplay between DNA polymerases beta and lambda in repair of oxidation DNA damage in chicken DT40 cells. DNA Repair (Amst) 6(6):869-875. PMID:17363341

2006

  • Chastain PD, Nakamura J, Swenberg JA, Kaufman DG. 2006. Nonrandom AP site distribution in highly proliferative cells. FASEB J 20(14):2612-4. PMID:17068113
  • Pachkowski BF, Winkel S, Kubota Y, Swenberg JA, Millikan RC, Nakamura J. 2006. XRCC1 genotype and breast cancer: functional studies and epidemiologic data show interactions between XRCC1 codon 280 His and smoking. Cancer Res 66(5):2860-2868. doi:10.1158/0008-5472.CAN-05-3388 PMID:16510609
  • Yoshimura M, Kohzaki M, Nakamura J, Asagoshi K, Sonoda E, Hou E, Prasad R, Wilson SH, Tano K, Yasui A, Lan L, Seki M, Wood RD, Arakawa H, Buerstedde J, Hochegger H, Okada T, Hiraoka M, Takeda S. 2006. Vertebrate POLQ and POLbeta cooperate in base excision repair of oxidative DNA damage. Mol Cell 24(1):115-125. doi:10.1016/j.molcel.2006.07.032 PMID:17018297 PMCID:PMC1868411

2005

  • Jeong Y, Nakamura J, Upton PB, Swenberg JA. 2005. Pyrimido[1,2-a]-purin-10(3H)-one, M1G, is less prone to artifact than base oxidation. Nucleic Acids Res 33(19):6426-6434. doi:10.1093/nar/gki944 PMID:16282591 PMCID:PMC1283527
  • Jeong Y, Sangaiah R, Nakamura J, Pachkowski BF, Ranasinghe A, Gold A, Ball LM, Swenberg JA. 2005. Analysis of M(1)G-dR in DNA by aldehyde reactive probe labeling and liquid chromatography tandem mass spectrometry. Chem Res Toxicol 18(1):51-60. PMID:15651849
  • Lin C, Leow H, Huang S, Nakamura J, Swenberg JA, Lin P. 2005. Induction of cytotoxicity, aldehydic DNA lesions, and poly(ADP-ribose) polymerase-1 activation by catechol derivatives of pentachlorophenol in calf thymus DNA and in human breast cancer cells. Chem Res Toxicol 18(2):257-264. PMID:15720130

2004

  • Zielinska-Park J, Nakamura J, Swenberg JA, Aitken MD. 2004. Aldehydic DNA lesions in calf thymus DNA and HeLa S3 cells produced by bacterial quinone metabolites of fluoranthene and pyrene. Carcinogenesis 25(9):1727-1733. PMID:15117810

2003

  • Barbin A, Ohgaki H, Nakamura J, Kurrer M, Kleihues P, Swenberg JA. 2003. Endogenous deoxyribonucleic acid (DNA) damage in human tissues: A comparison of ethenobases with aldehydic DNA lesions. Cancer Epidemiol Biomarkers Prev 12(11):1241-1247. PMID:14652288
  • Lin P, Nakamura J, Yamaguchi S, Asakura S, Swenberg JA. 2003. Aldehydic DNA lesions induced by catechol estrogens in calf thymus DNA. Carcinogenesis 24(6):1133-1141. PMID:12807746
  • Nakamura J, Asakura S, Hester SD, de Murcia G, Caldecott KW, Swenberg JA. 2003. Quantitation of intracellular NAD(P)H can monitor an imbalance of DNA single strand break repair in base excision repair deficient cells in real time. Nucleic Acids Res 31(17):e104. PMID:12930978
  • Nakamura J, Purvis ER, Swenberg JA. 2003. Micromolar concentrations of hydrogen peroxide induce oxidative DNA lesions more efficiently than millimolar concentrations in mammalian cells. Nucleic Acids Res 31(6):1790-1795. PMID:12626721
  • Nakamura J, Swenberg JA. 2003. Aldehyde Reactive Probe-Slot Blot Assay to quantitate DNA lesions caused by oxidative stress. In: Methods in Biological Oxidative Stress. Humana Press, pp.109-115.
  • Pachkowski BF, Nakamura J, Li Y, Sangaiah R, Gold A, Swenberg JA. 2003. An LC/MS Approach to the Differentiation of Aldehydic Deoxyribose Lesions. In: Proceedings of the American Association for Cancer Research Annual Meeting, 2003.

2002

  • Morinello EJ, Ham AL, Ranasinghe A, Nakamura J, Upton PB, Swenberg JA. 2002. Molecular dosimetry and repair of N2,3-ethenoguanine in rats exposed to vinyl chloride. Cancer Res 62:5189-5195. PMID:12234983
  • Sobol RW, Watson DE, Nakamura J, Yakes FM, Hou E, Horton JK, Wu J, Van Houten B, Swenberg JA, Tindall KR, Samson LD, Wilson SH. 2002. Mutations associated with base excision repair deficiency and methylation-induced genotoxic stress. Proc Natl Acad Sci U S A 99(14):6860-6865. PMID:11983862
  • Swenberg JA, Ham AL, McDorman KS, Morinello EJ, Nakamura J, Schoonhoven R. 2002. Methods for measuring DNA adducts and abasic sites I: isolation, purification, and analysis of DNA adducts in intact DNA. Curr Protoc Toxicol Chapter3:Unit3.8. doi:10.1002/0471140856.tx0308s12 PMID:23045079

2001

  • Lin P, Nakamura J, Yamaguchi S, La DK, Swenberg JA. 2001. Oxidative damage and direct adducts in calf thymus DNA induced by the pentachlorophenol metabolites, tetrachlorohydroquinone and tetrachloro-1,4-benzoquinone. Carcinogenesis 22(4):627-634. PMID:11285199
  • Lin P, Nakamura J, Yamaguchi S, La DK, Upton PB, Swenberg JA. 2001. Induction of direct adducts, apurinic/apyrimidinic sites and oxidized bases in nuclear DNA of human HeLa S3 tumor cells by tetrachlorohydroquinone. Carcinogenesis 22(4):635-639. PMID:11285200

2000

  • Nakamura J, La DK, Swenberg JA. 2000. 5'-Nicked apurinic/apyrimidinic sites are resistant to b-elimination by b-polymerase and are persistent in human cultured cells after oxidative stress. J Biol Chem 275(8):5323-5328.

1999

  • Ham AL, Ranasinghe A, Morinello EJ, Nakamura J, Upton PB, Johnson F, Swenberg JA. 1999. Immunoaffinity/gas chromatography/high resolution mass spectrometry method for the detection of N2,3-ethenoguanine. Chem Res Toxicol 12:1240-1246. PMID:10604874
  • Nakamura J. 1999. Determination of spontaneous depurination and endogenous AP sites in organs of F344 rats using ASB method. In: Proceedings of the Ninetieth Annual Meeting of the (AACR) American Association of Cancer Research, 1999. pp.648.
  • Nakamura J, Swenberg JA. 1999. Endogenous apurinic/apyrimidinic sites in genomic DNA of mammalian tissues. Cancer Res 59(11):2522-2526. PMID:10363965
  • Nakamura J, Walker V, Upton PB, Swenberg JA. 1999. Characterization of a newly developed AP site assay that uses an aldehyde reactive probe-Slot-Blot method. In: Proceedings of the Ninetieth Annual Meeting of the (AACR) American Association of Cancer Research, 1999. pp.480.

1998

  • Law JM, Bull M, Nakamura J, Swenberg JA. 1998. Molecular dosimetry of DNA adducts in the medaka small fish model. Carcinogenesis 19(3):515-518. PMID:9525288
  • Nakamura J, Walker V, Upton PB, Chiang S, Kow YW, Swenberg JA. 1998. Highly sensitive apurinic/apyrimidinic site assay can detect spontaneous and chemically induced depurination under physiological conditions. Cancer Res 58(2):222-225. PMID:9443396
  • Ploch SA, Nakamura J, Swenberg JA, Di Giulio RT. 1998. Assessment of AP site formation in isolated hepatocytes from brown bullhead upon treatment with benzo(a)pyrene. Fundam Appl Toxicol 42:82.
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