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Study Information
Study Attribute Value
CEBS Accession Number: 002-02128-0002-0000-7
Chemical Name: Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE)
CASRN: 54464-57-2
NTP Study Type: Genetic Toxicology - Micronucleus
NTP Study ID: F35719
Strain and Species: B6C3F1 Mouse
Study Result: Male: Equivocal; Female: Positive

Blood

Start Date Sample Collection Time Sex Methodology Used Route Dosing Regimen
2006-03-15 24 hour Male Flow Cytometry Dermal Dermal x 91, 13 week
  Dose
(%)		 Animal
Number		 Polychromatic Erythrocytes
Trend P: 0.865		 Normochromatic Erythrocytes
Trend P: 0.442
No. Examined Total MN Cells Percent PCE MN Cells
per 1000		 No. Examined Total MN Cells Percent NCE MN Cells
per 1000
Vehicle Control 95% Ethanol 
 0         
 11 20000 56 1.4 2.8 1400149 2089 98.6 1.5 
 0         
 12 20000 48 1.2 2.4 1704610 2510 98.8 1.5 
 0         
 13 20000 38 1.6 1.9 1213385 1816 98.4 1.5 
 0         
 14 20000 54 1.5 2.7 1333151 1995 98.5 1.5 
 0         
 15 20000 46 1.5 2.3 1309635 1839 98.5 1.4
Average ± SEM   1.455 ± 0.079 2.420 ± 0.160   0.000 ± 0.000 1.470 ± 0.020
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
6.25      
 21 20000 42 1.8 2.1 1098382 1742 98.2 1.6 
6.25      
 22 20000 44 1.6 2.2 1226444 1661 98.4 1.4 
6.25      
 23 20000 61 1.6 3.1 1208157 1692 98.4 1.4 
6.25      
 24 20000 64 1.4 3.2 1449530 2191 98.6 1.5 
6.25      
 25 20000 44 1.1 2.2 1764838 2712 98.9 1.5
Average ± SEM   1.524 ± 0.120 2.550 ± 0.240   0.000 ± 0.000 1.480 ± 0.040
Pairwise P   0.397   0.703
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
12.5       
 31 20000 48 1.3 2.4 1470667 1989 98.7 1.4 
12.5       
 32 20000 47 1.6 2.4 1207383 1654 98.4 1.4 
12.5       
 33 20000 44 1.5 2.2 1320033 1844 98.5 1.4 
12.5       
 34 20000 76 1.5 3.8 1295765 2028 98.5 1.6 
12.5       
 35 20000 63 1.8 3.2 1086847 1620 98.2 1.5
Average ± SEM   1.583 ± 0.080 2.780 ± 0.300   0.000 ± 0.000 1.430 ± 0.040
Pairwise P   0.471   0.787
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
25         
 41 20000 38 1.4 1.9 1380281 1613 98.6 1.2 
25         
 42 20000 52 1.5 2.6 1280219 1859 98.5 1.5 
25         
 43 20000 43 1.4 2.2 1429307 2056 98.6 1.4 
25         
 44 20000 49 1.6 2.5 1223394 1766 98.4 1.4 
25         
 45 20000 61 1.5 3.1 1295050 1856 98.5 1.4
Average ± SEM   1.518 ± 0.042 2.430 ± 0.200   0.000 ± 0.000 1.390 ± 0.050
Pairwise P   0.501   0.820
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
50         
 51 20000 42 1.4 2.1 1363585 2133 98.6 1.6 
50         
 52 20000 54 1.3 2.7 1561552 2274 98.7 1.5 
50         
 53 20000 42 1.6 2.1 1235246 1831 98.4 1.5 
50         
 54 20000 38 1.4 1.9 1362915 1960 98.6 1.4 
50         
 55 20000 48 1.5 2.4 1337829 2029 98.5 1.5
Average ± SEM   1.466 ± 0.054 2.240 ± 0.140   0.000 ± 0.000 1.490 ± 0.020
Pairwise P   0.518   0.477
 

Blood

Start Date Sample Collection Time Sex Methodology Used Route Dosing Regimen
2006-03-15 24 hour Male Flow Cytometry Dermal Dermal x 91, 13 week
  Dose
(%)		 Animal
Number		 Polychromatic Erythrocytes
Trend P: 		Normochromatic Erythrocytes
Trend P:
No. Examined Total MN Cells Percent PCE MN Cells
per 1000		 No. Examined Total MN Cells Percent NCE MN Cells
per 1000
Untreated Control 
 0         
 1 20000 56 1.40 2.80 1406028 2045 98.6 1.5 
 0         
 2 20000 51 1.74 2.55 1132598 1584 98.26 1.4 
 0         
 3 20000 62 1.50 3.10 1312591 1909 98.5 1.5 
 0         
 4 20000 45 1.59 2.25 1240589 1886 98.41 1.5 
 0         
 5 20000 52 1.64 2.60 1198744 1718 98.36 1.4
Average ± SEM   1.599 ± 0.059 2.660 ± 0.140   0.000 ± 0.000 1.450 ± 0.020
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
100         
 61 20000 39 1.7 2.0 1163708 1925 98.3 1.7 
100         
 62 20000 40 1.6 2.0 1227256 1913 98.4 1.6 
100         
 63 20000 51 2.6 2.6 739299 1115 97.4 1.5 
100         
 64 20000 45 1.8 2.3 1102237 1770 98.2 1.6 
100         
 65 20000 54 2.1 2.7 938813 1530 97.9 1.6
Average ± SEM   2.000 ± 0.196 2.290 ± 0.150   0.000 ± 0.000 1.590 ± 0.030
Pairwise P   0.805   0.001
 


Blood

Start Date Sample Collection Time Sex Methodology Used Route Dosing Regimen
2006-03-16 24 hour Female Flow Cytometry Dermal Dermal x 91, 13 week
  Dose
(%)		 Animal
Number		 Polychromatic Erythrocytes
Trend P: 0.856		 Normochromatic Erythrocytes
Trend P: <0.001
No. Examined Total MN Cells Percent PCE MN Cells
per 1000		 No. Examined Total MN Cells Percent NCE MN Cells
per 1000
Vehicle Control 95% Ethanol 
 0         
 81 20000 41 1.1 2.1 1792756 1843 98.9 1.0 
 0         
 82 20000 44 1.7 2.2 1163498 1213 98.3 1.0 
 0         
 83 20000 47 1.7 2.4 1126187 1174 98.3 1.0 
 0         
 84 20000 47 1.5 2.4 1321451 1332 98.5 1.0 
 0         
 85 20000 34 1.5 1.7 1345763 1262 98.5 0.9
Average ± SEM   1.522 ± 0.116 2.130 ± 0.120   0.000 ± 0.000 1.010 ± 0.020
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
6.25      
 91 20000 32 1.4 1.6 1426899 1397 98.6 1.0 
6.25      
 92 20000 37 1.5 1.9 1342201 1421 98.5 1.1 
6.25      
 93 20000 31 1.5 1.6 1308690 1321 98.5 1.0 
6.25      
 94 20000 34 1.8 1.7 1087918 1140 98.2 1.0 
6.25      
 95 20555 43 1.3 2.1 1590229 1552 98.7 1.0
Average ± SEM   1.510 ± 0.092 1.760 ± 0.100   0.000 ± 0.000 1.010 ± 0.020
Pairwise P   0.877   0.472
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
12.5       
 101 20000 43 2.2 2.2 882305 1046 97.8 1.2 
12.5       
 102 20000 41 1.8 2.1 1087758 1181 98.2 1.1 
12.5       
 103 20000 47 2.4 2.4 804409 741 97.6 0.9 
12.5       
 104 20000 41 1.8 2.1 1086082 1145 98.2 1.1 
12.5       
 105 20000 43 1.8 2.2 1072756 1187 98.2 1.1
Average ± SEM   2.060 ± 0.134 2.150 ± 0.050   0.000 ± 0.000 1.070 ± 0.040
Pairwise P   0.887   0.064
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
25         
 111 20000 32 2.1 1.6 955580 1043 97.9 1.1 
25         
 112 20000 37 1.4 1.9 1459242 1539 98.6 1.1 
25         
 113 20000 47 2.4 2.4 815140 927 97.6 1.1 
25         
 114 20000 35 1.9 1.8 1056361 1091 98.1 1.0 
25         
 115 20000 44 1.4 2.2 1430727 1555 98.6 1.1
Average ± SEM   1.842 ± 0.207 1.950 ± 0.140   0.000 ± 0.000 1.080 ± 0.020
Pairwise P   0.912   0.039
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
50         
 121 20000 33 2.1 1.7 916161 1016 97.9 1.1 
50         
 122 20000 34 1.6 1.7 1229374 1406 98.4 1.1 
50         
 123 20000 38 2.2 1.9 869556 1021 97.8 1.2 
50         
 124 20000 46 1.6 2.3 1223510 1375 98.4 1.1 
50         
 125 20997 36 2.0 1.7 1019573 1211 98.0 1.2
Average ± SEM   1.961 ± 0.140 1.850 ± 0.120   0.000 ± 0.000 1.150 ± 0.010
Pairwise P   0.924   0.001
 

Blood

Start Date Sample Collection Time Sex Methodology Used Route Dosing Regimen
2006-03-16 24 hour Female Flow Cytometry Dermal Dermal x 91, 13 week
  Dose
(%)		 Animal
Number		 Polychromatic Erythrocytes
Trend P: 		Normochromatic Erythrocytes
Trend P:
No. Examined Total MN Cells Percent PCE MN Cells
per 1000		 No. Examined Total MN Cells Percent NCE MN Cells
per 1000
Untreated Control 
 0         
 71 20000 36 1.16 1.80 1711476 1672 98.84 1.0 
 0         
 72 20000 42 1.11 2.10 1787821 1716 98.89 1.0 
 0         
 73 20000 50 1.54 2.50 1280589 1485 98.46 1.2 
 0         
 74 20404 35 0.73 1.72 2760118 2886 99.27 1.0 
 0         
 75 19999 36 0.78 1.80 2534803 2538 99.22 1.0
Average ± SEM   1.075 ± 0.149 1.980 ± 0.140   0.000 ± 0.000 1.030 ± 0.040
 
Test Chemical Ethanone, 1-(1,2,3,4,5,6,7,8-Octahydro-2,3,8,8-Tetramethyl-2-Naphthalenyl)- (Iso-E Super; OTNE) 
100         
 131 20000 44 0.9 2.2 2088522 2557 99.1 1.2 
100         
 132 20000 60 2.2 3.0 898373 1150 97.8 1.3 
100         
 133 20000 72 2.2 3.6 909579 1144 97.8 1.3 
100         
 134 20000 53 1.8 2.7 1090184 1406 98.2 1.3 
100         
 135 20000 59 2.2 3.0 889381 1160 97.8 1.3
Average ± SEM   1.893 ± 0.246 2.880 ± 0.230   0.000 ± 0.000 1.270 ± 0.010
Pairwise P   0.005   <0.001