Title: Repression of Ah receptor and induction of transforming growth factor-beta genes in DEN-induced mouse liver tumors.
Authors: Peng, Li; Mayhew, Christopher N; Schnekenburger, Michael; Knudsen, Erik S; Puga, Alvaro
Published In Toxicology, (2008 Apr 18)
Abstract: The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that mediates the biologic and toxic effects of its xenobiotic ligands. In recent years it has become evident that in the absence of ligand the AHR promotes cell cycle progression and that its activation by high-affinity ligands results in interactions with the retinoblastoma protein (RB) that lead to perturbation of the cell cycle, G0/G1 arrest, diminished capacity for DNA replication and inhibition of cell proliferation. Hence, the AHR has diametrically opposed pro-proliferative and anti-proliferative functions that have yet to be reconciled at the molecular level. Work from our own and from other laboratories suggests that the AHR may function as a tumor suppressor gene that becomes silenced in the process of tumor formation. To develop preliminary support for a more thorough examination of this hypothesis we characterized the expression levels of various tumor suppressor genes, transforming growth factor-beta (Tgfb) genes and the Ahr gene in liver tumor samples from mice with a liver-specific RB ablation and their wild-type littermates. In tumors arising in RB-positive livers, Cdkn2d and Tgfb1 were repressed and Cdkn2c, Tgfb2, Tgfb3 and Pai1 were induced, whereas in RB-negative tumors, only Cdkn2c and Tgfb3 were induced. Ahr was significantly repressed in tumors from both sets of mice, supporting the concept that Ahr silencing may be associated with cancer progression.
PubMed ID: 18282651
MeSH Terms: Animals; Base Sequence; Carcinogens/toxicity*; CpG Islands/drug effects; CpG Islands/genetics; DNA Methylation; DNA, Complementary/drug effects; DNA, Complementary/genetics; Diethylnitrosamine/toxicity*; Disease Models, Animal; Disease Progression; Gene Expression Regulation, Neoplastic; Gene Silencing*; Genes, Tumor Suppressor; Liver Neoplasms/chemically induced; Liver Neoplasms/genetics*; Liver Neoplasms/metabolism; Mice; Mice, Knockout; Molecular Sequence Data; Promoter Regions, Genetic/genetics; Receptors, Aryl Hydrocarbon/genetics*; Receptors, Aryl Hydrocarbon/metabolism; Retinoblastoma Protein/deficiency; Retinoblastoma Protein/genetics; Retinoblastoma Protein/metabolism; Transforming Growth Factor beta/genetics*; Transforming Growth Factor beta/metabolism