Title: Efficient purification of the biosurfactant viscosin from Pseudomonas libanensis strain M9-3 and its physicochemical and biological properties.

Authors: Saini, Harvinder S; Barragán-Huerta, Blanca E; Lebrón-Paler, Ariel; Pemberton, Jeanne E; Vázquez, Refugio R; Burns, Anna M; Marron, Marilyn T; Seliga, Chistopher J; Gunatilaka, A A Leslie; Maier, Raina M

Published In J Nat Prod, (2008 Jun)

Abstract: Viscosin (1), an effective surface-active cyclic lipopeptide, was efficiently recovered from Pseudomonas libanensis M9-3 with a simple purification protocol. A major pigment also obtained during this process was identified as phenazine-1-carboxylic acid. The critical micelle concentration (cmc) of viscosin was determined to be 54 mg L (-1), and the minimum surface tension between air and water at the cmc was 28 mN m (-1). Viscosin forms stable emulsions even at low concentrations (7.5 mg L (-1)), and the conditional stability constant for a cadmium-viscosin complex was determined to be 5.87. The physicochemical properties measured for viscosin are similar to other well-studied biosurfactants such as rhamnolipid and surfactin. Viscosin inhibited migration of the metastatic prostate cancer cell line, PC-3M, without visible toxicity. These properties suggest the potential of viscosin in environmental and biomedical applications.

PubMed ID: 18471020

MeSH Terms: Antineoplastic Agents/chemistry; Antineoplastic Agents/isolation & purification*; Antineoplastic Agents/pharmacology; Drug Screening Assays, Antitumor; Humans; Male; Molecular Structure; Peptides, Cyclic/chemistry; Peptides, Cyclic/isolation & purification*; Peptides, Cyclic/pharmacology*; Pseudomonas/chemistry*; Surface-Active Agents/chemistry; Surface-Active Agents/isolation & purification*; Surface-Active Agents/pharmacology*; Wound Healing/drug effects