Title: Formation, reactivity, and detection of protein sulfenic acids.

Authors: Kettenhofen, Nicholas J; Wood, Matthew J

Published In Chem Res Toxicol, (2010 Nov 15)

Abstract: It has become clear in recent decades that the post-translational modification of protein cysteine residues is a crucial regulatory event in biology. Evidence supports the reversible oxidation of cysteine thiol groups as a mechanism of redox-based signal transduction, while the accumulation of proteins with irreversible thiol oxidations is a hallmark of stress-induced cellular damage. The initial formation of cysteine-sulfenic acid (SOH) derivatives, along with the reactive properties of this functional group, serves as a crossroads whereby the local redox environment may dictate the progression of either regulatory or pathological outcomes. Protein-SOH are established as transient intermediates in the formation of more stable cysteine oxidation products both under basal conditions and in response to several redox-active extrinsic compounds. This review details both direct and multistep chemical routes proposed to generate protein-SOH, the spectrum of secondary reactions that may follow their initial formation and the arsenal of experimental tools available for their detection. Pioneering studies that have provided a framework for our current understanding of protein-SOH as well as state-of-the-art proteomic strategies designed for global assessments of this post-translational modification are highlighted.

PubMed ID: 20845928

MeSH Terms: Cysteine/chemistry; Oxidation-Reduction; Proteins/chemistry*; Proteomics; Signal Transduction; Sulfenic Acids/chemistry*; Sulfhydryl Compounds/chemistry