Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.


The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: Direct and specific chemical control of eukaryotic translation with a synthetic RNA-protein interaction.

Authors: Goldfless, Stephen J; Belmont, Brian J; de Paz, Alexandra M; Liu, Jessica F; Niles, Jacquin C

Published In Nucleic Acids Res, (2012 May)

Abstract: Sequence-specific RNA-protein interactions, though commonly used in biological systems to regulate translation, are challenging to selectively modulate. Here, we demonstrate the use of a chemically-inducible RNA-protein interaction to regulate eukaryotic translation. By genetically encoding Tet Repressor protein (TetR)-binding RNA elements into the 5'-untranslated region (5'-UTR) of an mRNA, translation of a downstream coding sequence is directly controlled by TetR and tetracycline analogs. In endogenous and synthetic 5'-UTR contexts, this system efficiently regulates the expression of multiple target genes, and is sufficiently stringent to distinguish functional from non-functional RNA-TetR interactions. Using a reverse TetR variant, we illustrate the potential for expanding the regulatory properties of the system through protein engineering strategies.

PubMed ID: 22275521 Exiting the NIEHS site

MeSH Terms: 5' Untranslated Regions*; Animals; Aptamers, Nucleotide/chemistry*; Aptamers, Nucleotide/metabolism; Cell-Free System; Gene Expression Regulation*; Polyribosomes/metabolism; Protein Biosynthesis*; Rabbits; Repressor Proteins/metabolism*; Saccharomyces cerevisiae/genetics

to Top