Title: Anti-aging effects of deuterium depletion on Mn-induced toxicity in a C. elegans model.
Authors: Avila, Daiana Silva; Somlyai, Gábor; Somlyai, Ildikó; Aschner, Michael
Published In Toxicol Lett, (2012 Jun 20)
Abstract: Work with sub-natural levels of deuterium (D) in animals has demonstrated an anti-cancer effect of low D-concentration in water. Our objective was to investigate whether deuterium-depleted water (DDW) can overturn reverse manganese (Mn)-induced reduction in life span, using the Caenorhabditis elegans (C. elegans) as a model system. DDW per se had no effect on worm's life span 48 h after treatment; however, it reversed the Mn-induced decrease in C. elegans life span. Mn reduced DAF-16 levels, a transcription factor strongly associated with life-span regulation. Low D-concentration (90 ppm) restored the Mn-induced changes in DAF-16 to levels indistinguishable from controls, suggesting DDW can regulate the DAF-16 pathway. We further show that insulin-like receptor DAF-2 levels were unaltered by Mn exposure, tAKT levels increased, whilst superoxide dismutase (SOD-3) levels were decreased by Mn. DDW (90 ppm) restored the levels of tAKT and superoxide dismutase (SOD) to control values without changing DAF-2 levels. Treatment of Mn exposed worms with DDW (90 ppm) restored life-span, DAF-16 and SOD-3 levels to control levels, strongly suggesting that low D concentrations can protect against Mn toxic effects.
PubMed ID: 22561170
MeSH Terms: Aging/drug effects*; Animals; Apoptosis/physiology; Blotting, Western; Caenorhabditis elegans Proteins/genetics; Caenorhabditis elegans Proteins/metabolism; Caenorhabditis elegans/physiology*; Deuterium/toxicity*; Forkhead Transcription Factors; Longevity/drug effects; Manganese Poisoning/pathology*; Proto-Oncogene Proteins c-akt/metabolism; Survival Analysis; Transcription Factors/metabolism; Water/chemistry