Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: A single neonatal exposure to aflatoxin b1 induces prolonged genetic damage in two loci of mouse liver.

Authors: Wattanawaraporn, Roongtiwa; Woo, Leslie L; Belanger, Crystal; Chang, Shiou-Chi; Adams, Jillian E; Trudel, Laura J; Bouhenguel, Jason T; Egner, Patricia A; Groopman, John D; Croy, Robert G; Essigmann, John M; Wogan, Gerald N

Published In Toxicol Sci, (2012 Aug)

Abstract: Aflatoxin B (1) (AFB(1)) is a risk factor for hepatocellular carcinoma in humans. Infant, but not adult, mice are sensitive to AFB(1)-induced liver carcinogenesis; a single dose during the neonatal period leads to hepatocellular carcinoma in adulthood. Earlier work defined the mutational spectrum in the gpt gene of gpt delta B6C3F1 mice 3 weeks after exposure to aflatoxin. In the present study, we examined the gpt spectrum 10 weeks postdosing and expanded the study to examine, at 3 and 10 weeks, the spectrum at a second locus, the red/gam genes of the mouse λEG10 transgene. Whereas the gpt locus is typically used to define local base changes, the red/gam genes, via the Spi(-) assay, often are used to detect more global mutations such as large deletions and rearrangements. Three weeks after dosing with AFB(1), there was a 10-fold increase over the control in the Spi(-) mutant fraction (MF) in liver DNA; after 10 weeks, a further increase was observed. The MF in the gpt gene was also increased at 10 weeks compared with the MF at 3 weeks. No gender-specific differences were found in the Spi(-) or gpt MFs. Whereas Spi(-) mutations often signal large genetic changes, they did not in this specific case. The Spi(-) spectrum was dominated by GC to TA transversions, with one exceptionally strong hotspot at position 314. Using two genetic loci, the data show a strong preference for the induction of GC to TA mutations in mice, which is the dominant mutation seen in people exposed to aflatoxin.

PubMed ID: 22539618 Exiting the NIEHS site

MeSH Terms: Aflatoxin B1/administration & dosage; Aflatoxin B1/toxicity*; Animals; Animals, Newborn; Base Sequence; DNA Primers; Dose-Response Relationship, Drug; Liver/drug effects*; Male; Mice; Mice, Inbred C3H; Polymerase Chain Reaction

Back
to Top