Title: Preparation of 20-HETE using multifunctional enzyme type 2-negative Starmerella bombicola.

Authors: Van Bogaert, Inge; Zhang, Guodong; Yang, Jun; Liu, Jun-Yan; Ye, Yonghao; Soetaert, Wim; Hammock, Bruce D

Published In J Lipid Res, (2013 Nov)

Abstract: The metabolism of arachidonic acid (ARA) by cytochrome P450 ω/ω-1-hydroxylases leads to the formation of 20-hydroxyeicosatetraenoic acid (20-HETE), which is an important lipid-signaling molecule involved in regulation of vascular tone, angiogenesis, and inflammation. Development of a simple method to prepare 20-HETE would greatly facilitate the investigation of its biological activities. The nonpathogenic yeast Starmerella bombicola has been shown to convert exogenously added arachidonic acid to 20-HETE via the biosynthetic pathway of sophorolipids; however, the yield was low. Here we demonstrate that genetic knockout of multifunctional enzyme type 2 (MFE-2), which is involved in the β-oxidation of fatty acids, significantly increases the yield of ARA conversion to 20-HETE and allows practical preparation of 20-HETE.

PubMed ID: 23966667

MeSH Terms: Animals; Arachidonic Acid/metabolism; Ascomycota/enzymology*; Cytochrome P-450 Enzyme System/metabolism; Gene Knockout Techniques*; Hydroxyeicosatetraenoic Acids/biosynthesis*; Hydroxyeicosatetraenoic Acids/metabolism; Hydroxyeicosatetraenoic Acids/pharmacology; Hydroxylation; Hydroxysteroid Dehydrogenases/deficiency*; Hydroxysteroid Dehydrogenases/genetics*; Hydroxysteroid Dehydrogenases/metabolism; Mice; Neovascularization, Physiologic/drug effects; Oxidation-Reduction