Title: Loss of regulator of G protein signaling 5 promotes airway hyperresponsiveness in the absence of allergic inflammation.
Authors: Balenga, Nariman A; Jester, William; Jiang, Meiqi; Panettieri Jr, Reynold A; Druey, Kirk M
Published In J Allergy Clin Immunol, (2014 Aug)
Abstract: Although eosinophilic inflammation typifies allergic asthma, it is not a prerequisite for airway hyperresponsiveness (AHR), suggesting that underlying abnormalities in structural cells, such as airway smooth muscle (ASM), contribute to the asthmatic diathesis. Dysregulation of procontractile G protein-coupled receptor (GPCR) signaling in ASM could mediate enhanced contractility.We explored the role of a regulator of procontractile GPCR signaling, regulator of G protein signaling 5 (RGS5), in unprovoked and allergen-induced AHR.We evaluated GPCR-evoked Ca(2+) signaling, precision-cut lung slice (PCLS) contraction, and lung inflammation in naive and Aspergillus fumigatus-challenged wild-type and Rgs5(-/-) mice. We analyzed lung resistance and dynamic compliance in live anesthetized mice using invasive plethysmography.Loss of RGS5 promoted constitutive AHR because of enhanced GPCR-induced Ca(2+) mobilization in ASM. PCLSs from naive Rgs5(-/-) mice contracted maximally at baseline independently of allergen challenge. RGS5 deficiency had little effect on the parameters of allergic inflammation, including cell counts in bronchoalveolar lavage fluid, mucin production, ASM mass, and subepithelial collagen deposition. Unexpectedly, induced IL-13 and IL-33 levels were much lower in challenged lungs from Rgs5(-/-) mice relative to those seen in wild-type mice.Loss of RGS5 confers spontaneous AHR in mice in the absence of allergic inflammation. Because it is selectively expressed in ASM within the lung and does not promote inflammation, RGS5 might be a therapeutic target for asthma.
PubMed ID: 24666695
MeSH Terms: Allergens/administration & dosage; Allergens/immunology*; Animals; Aspergillus fumigatus/immunology; Bronchoalveolar Lavage Fluid/immunology; Calcium Signaling; Calcium/immunology*; Female; Gene Expression Regulation; Injections, Intraperitoneal; Interleukin-13/genetics; Interleukin-13/immunology; Interleukin-33; Interleukins/genetics; Interleukins/immunology; Lung/drug effects; Lung/immunology; Lung/pathology*; Mice; Mice, Knockout; Mucins/immunology; Muscle Contraction; Myocytes, Smooth Muscle/immunology; Myocytes, Smooth Muscle/pathology*; Plethysmography; RGS Proteins/deficiency; RGS Proteins/genetics; RGS Proteins/immunology*; Respiratory Hypersensitivity/genetics; Respiratory Hypersensitivity/immunology; Respiratory Hypersensitivity/pathology*; Respiratory Mucosa; Tissue Culture Techniques