Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.


The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: Ambient fine particulate matter induces apoptosis of endothelial progenitor cells through reactive oxygen species formation.

Authors: Cui, Yuqi; Xie, Xiaoyun; Jia, Fengpeng; He, Jianfeng; Li, Zhihong; Fu, Minghuan; Hao, Hong; Liu, Ying; Liu, Jason Z; Cowan, Peter J; Zhu, Hua; Sun, Qinghua; Liu, Zhenguo

Published In Cell Physiol Biochem, (2015)

Abstract: Bone marrow (BM)-derived endothelial progenitor cells (EPCs) play a critical role in angiogenesis and vascular repair. Some environmental insults, like fine particulate matter (PM) exposure, significantly impair cardiovascular functions. However, the mechanisms for PM-induced adverse effects on cardiovascular system remain largely unknown. The present research was to study the detrimental effects of PM on EPCs and explore the potential mechanisms.PM was intranasal-distilled into male C57BL/6 mice for one month. Flow cytometry was used to measure the number of EPCs, apoptosis level of circulating EPCs and intracellular reactive oxygen species (ROS) formation. Serum TNF-α and IL-1β were measured using ELISA. To determine the role of PM-induced ROS in EPC apoptosis, PM was co-administrated with the antioxidant N-acetylcysteine (NAC) in wild type mice or used in a triple transgenic mouse line (TG) with overexpression of antioxidant enzyme network (AON) composed of superoxide dismutase (SOD)1, SOD3, and glutathione peroxidase (Gpx-1) with decreased in vivo ROS production.PM treatment significantly decreased circulating EPC population, promoted apoptosis of EPCs in association with increased ROS production and serum TNF-α and IL-1β levels, which could be effectively reversed by either NAC treatment or overexpression of AON.PM exposure significantly decreased circulating EPCs population due to increased apoptosis via ROS formation in mice.

PubMed ID: 25591776 Exiting the NIEHS site

MeSH Terms: Acetylcysteine/pharmacology; Animals; Apoptosis/drug effects*; Bone Marrow Cells/cytology; Cells, Cultured; Endothelial Progenitor Cells/cytology; Endothelial Progenitor Cells/drug effects; Endothelial Progenitor Cells/metabolism; Enzyme-Linked Immunosorbent Assay; Glutathione Peroxidase/genetics; Glutathione Peroxidase/metabolism; Interleukin-1beta/blood; Lung/metabolism; Lung/pathology; Male; Mice; Mice, Inbred C57BL; Particulate Matter/toxicity*; Reactive Oxygen Species/metabolism*; Superoxide Dismutase-1; Superoxide Dismutase/genetics; Superoxide Dismutase/metabolism; Tumor Necrosis Factor-alpha/blood

to Top