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National Institute of Environmental Health Sciences

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Publication Detail

Title: A microfluidic device for epigenomic profiling using 100 cells.

Authors: Cao, Zhenning; Chen, Changya; He, Bing; Tan, Kai; Lu, Chang

Published In Nat Methods, (2015 Oct)

Abstract: The sensitivity of chromatin immunoprecipitation (ChIP) assays poses a major obstacle for epigenomic studies of low-abundance cells. Here we present a microfluidics-based ChIP-seq protocol using as few as 100 cells via drastically improved collection of high-quality ChIP-enriched DNA. Using this technology, we uncovered many new enhancers and super enhancers in hematopoietic stem and progenitor cells from mouse fetal liver, suggesting that enhancer activity is highly dynamic during early hematopoiesis.

PubMed ID: 26214128 Exiting the NIEHS site

MeSH Terms: Acetylation; Animals; Chromatin Immunoprecipitation/instrumentation*; Chromatin Immunoprecipitation/methods*; Enhancer Elements, Genetic; Epigenomics/instrumentation*; Epigenomics/methods; Equipment Design; Female; Hematopoiesis; Hematopoietic Stem Cells/physiology; Histones/metabolism; Humans; Lab-On-A-Chip Devices*; Liver/embryology; Liver/physiology; Male; Manufacturing Industry/methods; Mice, Inbred C57BL

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