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Publication Detail

Title: Quantification of Glutathione in Caenorhabditis elegans.

Authors: Caito, Samuel W; Aschner, Michael

Published In Curr Protoc Toxicol, (2015)

Abstract: Glutathione (GSH) is the most abundant intracellular thiol with diverse functions from redox signaling, xenobiotic detoxification, and apoptosis. The quantification of GSH is an important measure for redox capacity and oxidative stress. This protocol quantifies total GSH from Caenorhabditis elegans, an emerging model organism for toxicology studies. GSH is measured using the 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) cycling method originally created for cell and tissue samples but optimized for whole worm extracts. DTNB reacts with GSH to from a 5'-thio-2-nitrobenzoic acid (TNB) chromophore with maximum absorbance of 412 nm. This method is both rapid and sensitive, making it ideal for studies involving a large number of transgenic nematode strains.

PubMed ID: 26309452 Exiting the NIEHS site

MeSH Terms: Animals; Caenorhabditis elegans/drug effects; Caenorhabditis elegans/metabolism*; Dithionitrobenzoic Acid/toxicity*; Glutathione/analysis*; Glutathione/metabolism; Oxidative Stress/drug effects; Sensitivity and Specificity; Spectrophotometry; Toxicity Tests/methods*

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