Title: Spatially-resolved intracellular sensing of hydrogen peroxide in living cells.

Authors: Warren, Emilie A K; Netterfield, Tatiana S; Sarkar, Saheli; Kemp, Melissa L; Payne, Christine K

Published In Sci Rep, (2015 Nov 20)

Abstract: Understanding intracellular redox chemistry requires new tools for the site-specific visualization of intracellular oxidation. We have developed a spatially-resolved intracellular sensor of hydrogen peroxide, HyPer-Tau, for time-resolved imaging in live cells. This sensor consists of a hydrogen peroxide-sensing protein tethered to microtubules. We demonstrate the use of the HyPer-Tau sensor for three applications; dose-dependent response of human cells to exogenous hydrogen peroxide, a model immune response of mouse macrophages to stimulation by bacterial toxin, and a spatially-resolved response to localized delivery of hydrogen peroxide. These results demonstrate that HyPer-Tau can be used as an effective tool for tracking changes in spatially localized intracellular hydrogen peroxide and for future applications in redox signaling.

PubMed ID: 26585385

MeSH Terms: Animals; Biosensing Techniques/methods*; Cell Line; HeLa Cells; Humans; Hydrogen Peroxide/metabolism*; Hydrogen Peroxide/pharmacology; Intracellular Space/drug effects; Intracellular Space/metabolism*; Lipopolysaccharides/pharmacology; Macrophages/cytology; Macrophages/drug effects; Macrophages/metabolism*; Mice; Microscopy, Confocal; Microscopy, Fluorescence; Oxidants/metabolism; Oxidants/pharmacology; Oxidation-Reduction; Plasmids/genetics; Plasmids/metabolism; Time-Lapse Imaging/methods; tau Proteins/genetics; tau Proteins/metabolism