Skip Navigation
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.


The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Your Environment. Your Health.

Publication Detail

Title: Tether mutations that restore function and suppress pleiotropic phenotypes of the C. elegans isp-1(qm150) Rieske iron-sulfur protein.

Authors: Jafari, Gholamali; Wasko, Brian M; Tonge, Ashley; Schurman, Nathan; Dong, Cindy; Li, Zhongyu; Peters, Rebecca; Kayser, Ernst-Bernhard; Pitt, Jason N; Morgan, Phil G; Sedensky, Margaret M; Crofts, Antony R; Kaeberlein, Matt

Published In Proc Natl Acad Sci U S A, (2015 Nov 10)

Abstract: Mitochondria play an important role in numerous diseases as well as normative aging. Severe reduction in mitochondrial function contributes to childhood disorders such as Leigh Syndrome, whereas mild disruption can extend the lifespan of model organisms. The Caenorhabditis elegans isp-1 gene encodes the Rieske iron-sulfur protein subunit of cytochrome c oxidoreductase (complex III of the electron transport chain). The partial loss of function allele, isp-1(qm150), leads to several pleiotropic phenotypes. To better understand the molecular mechanisms of ISP-1 function, we sought to identify genetic suppressors of the delayed development of isp-1(qm150) animals. Here we report a series of intragenic suppressors, all located within a highly conserved six amino acid tether region of ISP-1. These intragenic mutations suppress all of the evaluated isp-1(qm150) phenotypes, including developmental rate, pharyngeal pumping rate, brood size, body movement, activation of the mitochondrial unfolded protein response reporter, CO2 production, mitochondrial oxidative phosphorylation, and lifespan extension. Furthermore, analogous mutations show a similar effect when engineered into the budding yeast Rieske iron-sulfur protein Rip1, revealing remarkable conservation of the structure-function relationship of these residues across highly divergent species. The focus on a single subunit as causal both in generation and in suppression of diverse pleiotropic phenotypes points to a common underlying molecular mechanism, for which we propose a "spring-loaded" model. These observations provide insights into how gating and control processes influence the function of ISP-1 in mediating pleiotropic phenotypes including developmental rate, movement, sensitivity to stress, and longevity.

PubMed ID: 26504246 Exiting the NIEHS site

MeSH Terms: Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins/chemistry*; Caenorhabditis elegans Proteins/genetics*; Caenorhabditis elegans Proteins/physiology; Clutch Size/genetics; Electron Transport Complex III/chemistry*; Electron Transport Complex III/genetics*; Electron Transport Complex III/physiology; Genetic Pleiotropy/genetics*; Growth and Development/genetics; Longevity/genetics; Microscopy, Fluorescence; Models, Molecular*; Movement/physiology; Mutagenesis; Mutation/genetics; Nuclear Pore Complex Proteins/genetics; Phenotype*; Protein Engineering; Saccharomyces cerevisiae Proteins/genetics; Stress, Physiological/genetics

to Top