Title: Integrated Proteomic and Genomic Analysis of Gastric Cancer Patient Tissues.
Authors: Yan, Julia Fangfei; Kim, Hoguen; Jeong, Seul-Ki; Lee, Hyoung-Joo; Sethi, Manveen K; Lee, Ling Y; Beavis, Ronald C; Im, Hogune; Snyder, Michael P; Hofree, Matan; Ideker, Trey; Wu, Shiaw-Lin; Paik, Young-Ki; Fanayan, Susan; Hancock, William S
Published In J Proteome Res, (2015 Dec 04)
Abstract: V-erb-b2 erythroblastic leukemia viral oncogene homologue 2, known as ERBB2, is an important oncogene in the development of certain cancers. It can form a heterodimer with other epidermal growth factor receptor family members and activate kinase-mediated downstream signaling pathways. ERBB2 gene is located on chromosome 17 and is amplified in a subset of cancers, such as breast, gastric, and colon cancer. Of particular interest to the Chromosome-Centric Human Proteome Project (C-HPP) initiative is the amplification mechanism that typically results in overexpression of a set of genes adjacent to ERBB2, which provides evidence of a linkage between gene location and expression. In this report we studied patient samples from ERBB2-positive together with adjacent control nontumor tissues. In addition, non-ERBB2-expressing patient samples were selected as comparison to study the effect of expression of this oncogene. We detected 196 proteins in ERBB2-positive patient tumor samples that had minimal overlap (29 proteins) with the non-ERBB2 tumor samples. Interaction and pathway analysis identified extracellular signal regulated kinase (ERK) cascade and actin polymerization and actinmyosin assembly contraction as pathways of importance in ERBB2+ and ERBB2- gastric cancer samples, respectively. The raw data files are deposited at ProteomeXchange (identifier: PXD002674) as well as GPMDB.
PubMed ID: 26435392
MeSH Terms: Case-Control Studies; Cell Line, Tumor; Gene Expression Profiling; Humans; Immunohistochemistry; In Situ Hybridization, Fluorescence; Receptor, ErbB-2/metabolism*; Stomach Neoplasms/genetics*; Stomach Neoplasms/metabolism*