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National Institute of Environmental Health Sciences

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Publication Detail

Title: Quantitative Proteomic Analysis of Serum Exosomes from Patients with Locally Advanced Pancreatic Cancer Undergoing Chemoradiotherapy.

Authors: An, Mingrui; Lohse, Ines; Tan, Zhijing; Zhu, Jianhui; Wu, Jing; Kurapati, Himabindu; Morgan, Meredith A; Lawrence, Theodore S; Cuneo, Kyle C; Lubman, David M

Published In J Proteome Res, (2017 Apr 07)

Abstract: Pancreatic cancer is the third leading cause of cancer-related death in the USA. Despite extensive research, minimal improvements in patient outcomes have been achieved. Early identification of treatment response and metastasis would be valuable to determine the appropriate therapeutic course for patients. In this work, we isolated exosomes from the serum of 10 patients with locally advanced pancreatic cancer at serial time points over a course of therapy, and quantitative analysis was performed using the iTRAQ method. We detected approximately 700-800 exosomal proteins per sample, several of which have been implicated in metastasis and treatment resistance. We compared the exosomal proteome of patients at different time points during treatment to healthy controls and identified eight proteins that show global treatment-specific changes. We then tested the effect of patient-derived exosomes on the migration of tumor cells and found that patient-derived exosomes, but not healthy controls, induce cell migration, supporting their role in metastasis. Our data show that exosomes can be reliably extracted from patient serum and analyzed for protein content. The differential loading of exosomes during a course of therapy suggests that exosomes may provide novel insights into the development of treatment resistance and metastasis.

PubMed ID: 28240915 Exiting the NIEHS site

MeSH Terms: Blood Proteins/biosynthesis; Blood Proteins/genetics*; Exosomes/drug effects; Exosomes/radiation effects; Female; Gene Expression Regulation, Neoplastic/drug effects; Gene Expression Regulation, Neoplastic/radiation effects; Humans; Male; Neoplasm Recurrence, Local/blood*; Neoplasm Recurrence, Local/drug therapy; Neoplasm Recurrence, Local/pathology; Neoplasm Recurrence, Local/radiotherapy; Pancreatic Neoplasms/blood*; Pancreatic Neoplasms/drug therapy; Pancreatic Neoplasms/pathology; Pancreatic Neoplasms/radiotherapy; Proteome/genetics*

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