Title: Imprinted gene dysregulation in a Tet1 null mouse model is stochastic and variable in the germline and offspring.
Authors: SanMiguel, Jennifer M; Abramowitz, Lara K; Bartolomei, Marisa S
Published In Development, (2018 03 29)
Abstract: Imprinted genes are expressed from one parental allele and regulated by differential DNA methylation at imprinting control regions (ICRs). ICRs are reprogrammed in the germline through erasure and re-establishment of DNA methylation. Although much is known about DNA methylation establishment, DNA demethylation is less well understood. Recently, the Ten-Eleven Translocation proteins (TET1-3) have been shown to initiate DNA demethylation, with Tet1-/- mice exhibiting aberrant levels of imprinted gene expression and ICR methylation. Nevertheless, the role of TET1 in demethylating ICRs in the female germline and in controlling allele-specific expression remains unknown. Here, we examined ICR-specific DNA methylation in Tet1-/- germ cells and ascertained whether abnormal ICR methylation impacted imprinted gene expression in F1 hybrid somatic tissues derived from Tet1-/- eggs or sperm. We show that Tet1 deficiency is associated with hypermethylation of a subset of ICRs in germ cells. Moreover, ICRs with defective germline reprogramming exhibit aberrant DNA methylation and biallelic expression of linked imprinted genes in somatic tissues. Thus, we define a discrete set of genomic regions that require TET1 for germline reprogramming and discuss mechanisms for stochastic imprinting defects.
PubMed ID: 29530881
MeSH Terms: Animals; DNA Methylation/genetics*; DNA-Binding Proteins/genetics*; Epigenomics; Female; Genomic Imprinting/genetics*; Germ Cells/metabolism*; Male; Mice; Mice, Inbred C57BL; Mice, Inbred ICR; Mice, Knockout; Proto-Oncogene Proteins/genetics*; Real-Time Polymerase Chain Reaction