Title: Knockout of human arylamine N-acetyltransferase 1 (NAT1) in MDA-MB-231 breast cancer cells leads to increased reserve capacity, maximum mitochondrial capacity, and glycolytic reserve capacity.

Authors: Carlisle, Samantha M; Trainor, Patrick J; Doll, Mark A; Stepp, Marcus W; Klinge, Carolyn M; Hein, David W

Published In Mol Carcinog, (2018 11)

Abstract: Human arylamine N-acetyltransferase 1 (NAT1) is a phase II xenobiotic metabolizing enzyme found in almost all tissues. NAT1 can also hydrolyze acetyl-coenzyme A (acetyl-CoA) in the absence of an arylamine substrate. Expression of NAT1 varies between individuals and is elevated in several cancers including estrogen receptor positive (ER+) breast cancers. To date, however, the exact mechanism by which NAT1 expression affects mitochondrial bioenergetics in breast cancer cells has not been described. To further evaluate the role of NAT1 in energy metabolism MDA-MB-231 breast cancer cells with parental, increased, and knockout levels of NAT1 activity were compared for bioenergetics profile. Basal oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured followed by programmed sequential injection of Oligomycin (ATP synthase inhibitor), FCCP (ETC uncoupler), Antimycin A (Complex III inhibitor), and Rotenone (Complex I inhibitor) to evaluate mitochondrial bioenergetics. Compared to the cell lines with parental NAT1 activity, NAT1 knockout MDA-MB-231 cell lines exhibited significant differences in bioenergetics profile, while those with increased NAT1 did not. Significant increases in reserve capacity, maximum mitochondrial capacity, and glycolytic reserve capacity were observed in NAT1 knockout MDA-MB-231 cell lines compared to those with parental and increased NAT1 activity. These data indicate that NAT1 knockout in MDA-MB-231 breast cancer cells may enhance adaptation to stress by increasing plasticity in response to energy demand.

PubMed ID: 29964355

MeSH Terms: Arylamine N-Acetyltransferase/genetics*; Arylamine N-Acetyltransferase/metabolism; Breast Neoplasms/genetics; Breast Neoplasms/metabolism; Breast Neoplasms/mortality; Breast Neoplasms/pathology; Cell Line, Tumor; Female; Gene Expression Regulation, Neoplastic; Gene Knockout Techniques; Glycolysis; Humans; Isoenzymes/genetics*; Isoenzymes/metabolism; Mitochondria/genetics*; Mitochondria/metabolism*; Organelle Biogenesis; Oxygen Consumption; Transcription, Genetic