Title: Assaying Oxidative Coupling Activity of CYP450 Enzymes.
Authors: Agarwal, Vinayak
Published In Methods Enzymol, (2018)
Abstract: Cytochrome P450 (CYP450) enzymes are ubiquitous catalysts in natural product biosynthetic schemes where they catalyze numerous different transformations using radical intermediates. In this protocol, we describe procedures to assay the activity of a marine bacterial CYP450 enzyme Bmp7 which catalyzes the oxidative radical coupling of polyhalogenated aromatic substrates. The broad substrate tolerance of Bmp7, together with rearrangements of the aryl radical intermediates leads to a large number of products to be generated by the enzymatic action of Bmp7. The complexity of the product pool generated by Bmp7 thus presents an analytical challenge for structural elucidation. To address this challenge, we describe mass spectrometry-based procedures to provide structural insights into aryl crosslinked products generated by Bmp7, which can complement subsequent spectroscopic experiments. Using the procedures described here, for the first time, we show that Bmp7 can efficiently accept polychlorinated aryl substrates, in addition to the physiological polybrominated substrates for the biosynthesis of polyhalogenated marine natural products.
PubMed ID: 29779665
MeSH Terms: Biochemistry/instrumentation; Biochemistry/methods*; Cytochrome P-450 Enzyme System/chemistry*; Cytochrome P-450 Enzyme System/genetics; Cytochrome P-450 Enzyme System/metabolism*; Gammaproteobacteria/enzymology; Gammaproteobacteria/genetics; Mass Spectrometry/methods; Oxidative Coupling*; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Substrate Specificity