Title: Single-cell transcriptomic analysis of mouse neocortical development.

Authors: Loo, Lipin; Simon, Jeremy M; Xing, Lei; McCoy, Eric S; Niehaus, Jesse K; Guo, Jiami; Anton, E S; Zylka, Mark J

Published In Nat Commun, (2019 Jan 11)

Abstract: The development of the mammalian cerebral cortex depends on careful orchestration of proliferation, maturation, and migration events, ultimately giving rise to a wide variety of neuronal and non-neuronal cell types. To better understand cellular and molecular processes that unfold during late corticogenesis, we perform single-cell RNA-seq on the mouse cerebral cortex at a progenitor driven phase (embryonic day 14.5) and at birth-after neurons from all six cortical layers are born. We identify numerous classes of neurons, progenitors, and glia, their proliferative, migratory, and activation states, and their relatedness within and across age. Using the cell-type-specific expression patterns of genes mutated in neurological and psychiatric diseases, we identify putative disease subtypes that associate with clinical phenotypes. Our study reveals the cellular template of a complex neurodevelopmental process, and provides a window into the cellular origins of brain diseases.

PubMed ID: 30635555

MeSH Terms: Animals; Base Sequence; Cell Line; Cell Lineage/genetics*; Ciliopathies/genetics; Embryonic Development/genetics*; Female; Gene Expression Profiling*; HEK293 Cells; Humans; Male; Mental Disorders/genetics; Mice; Mice, Inbred C57BL; Neocortex/embryology*; Neurodevelopmental Disorders/genetics; Neurons/cytology; Neurons/metabolism; Sequence Analysis, RNA; Single-Cell Analysis; Stem Cells/cytology; Transcription, Genetic/genetics