Title: Pseudomonas sp. strain KC represents a new genomovar within Pseudomonas stutzeri.

Authors: Sepúlveda-Torres, L C; Zhou, J; Guasp, C; Lalucat, J; Knaebel, D; Plank, J L; Criddle, C S

Published In Int J Syst Evol Microbiol, (2001 Nov)

Abstract: Pseudomonas sp. strain KC (= ATCC 55595 = DSM 7136) is a denitrifying aquifer isolate that produces and secretes pyridine-2,6-bis(thiocarboxylate) (PDTC), a chelating agent that fortuitously transforms carbon tetrachloride without producing chloroform. Although KC has been used successfully for full-scale bioremediation of carbon tetrachloride, its taxonomy has proven difficult to resolve, as it retains properties of both Pseudomonas stutzeri and Pseudomonas putida. In the present work, a polyphasic approach was used to conclude that strain KC represents a new genomovar (genomovar 9) within the species P. stutzeri.

PubMed ID: 11760942

MeSH Terms: Biodegradation, Environmental; Carbon Tetrachloride/metabolism*; DNA Gyrase/genetics; DNA, Ribosomal/genetics; Fresh Water/microbiology; Inositol/metabolism; Molecular Sequence Data; Nitrites/metabolism; Nucleic Acid Hybridization; Phenotype; Phylogeny*; Polymerase Chain Reaction; Pseudomonas/classification*; Pseudomonas/genetics*; Pseudomonas/metabolism; Pyridines/chemistry; Pyridines/metabolism*; RNA, Ribosomal, 16S/genetics; Sequence Analysis, DNA