Title: In utero exposure to diesel exhaust is associated with alterations in neonatal cardiomyocyte transcription, DNA methylation and metabolic perturbation.
Authors: Goodson, Jamie M; MacDonald, James W; Bammler, Theo K; Chien, Wei-Ming; Chin, Michael T
Published In Part Fibre Toxicol, (2019 04 11)
Abstract: Developmental exposure to particulate matter air pollution is harmful to cardiovascular health, but the mechanisms by which this exposure mediates susceptibility to heart disease is poorly understood. We have previously shown, in a mouse model, that gestational exposure to diesel exhaust (DE) results in increased cardiac hypertrophy, fibrosis and susceptibility to heart failure in the adult offspring following transverse aortic constriction.In this study, we have analyzed gene expression in neonatal cardiomyocytes after gestational exposure by RNA-sequencing and have identified 300 genes that are dysregulated, including many involved in cardiac metabolism. We subsequently determined that these cardiomyocytes exhibit reduced metabolic activity as measured by Seahorse extracellular flux analysis. We also surveyed for modifications in DNA methylation at global regulatory regions using reduced representation bisulfite sequencing and found hypomethylation of DNA in neonatal cardiomyocytes isolated from in utero DE exposed neonates.We have demonstrated that in utero exposure to diesel exhaust alters the neonatal cardiomyocyte transcriptional and epigenetic landscapes, as well as the metabolic capability of these cells. Understanding how exposure alters the developing heart through dysregulation of gene expression, metabolism and DNA methylation is vital for identifying therapeutic interventions for air pollution-related heart failure.
PubMed ID: 30975218
MeSH Terms: Animals; Animals, Newborn; DNA Methylation/drug effects*; Female; Inhalation Exposure/adverse effects; Maternal Exposure/adverse effects; Metabolome/drug effects*; Mice, Inbred C57BL; Myocytes, Cardiac/drug effects*; Myocytes, Cardiac/metabolism; Particulate Matter/toxicity*; Pregnancy; Prenatal Exposure Delayed Effects/chemically induced*; Prenatal Exposure Delayed Effects/genetics; Prenatal Exposure Delayed Effects/metabolism; Transcriptome/drug effects*; Vehicle Emissions/toxicity*