Title: Endocrine-disrupting chemicals alter the neuromolecular phenotype in F2 generation adult male rats.
Authors: Krishnan, Krittika; Hasbum, Asbiel; Morales, Daniel; Thompson, Lindsay M; Crews, David; Gore, Andrea C
Published In Physiol Behav, (2019 11 01)
Abstract: Endocrine-disrupting chemical (EDC) exposures to the fetus have long-lasting effects on health and disease in adulthood. Such EDC exposure to the F1 fetuses also reaches the germ cells that become the F2 generation. Previously, we demonstrated that adult social and communicative behaviors such as ultrasonic vocalizations and mating behaviors were altered by EDCs in F2 rats, especially males. In the current study, we used the brains of these F2 males to ascertain the underlying molecular changes in the hypothalamus related to these behavioral outcomes. Their progenitors were Sprague-Dawley rat dams, treated on pregnancy days 8 to 18 with one of three treatments: a polychlorinated biphenyl (PCB) mixture, Aroclor 1221, selected because it is weakly estrogenic; the anti-androgenic fungicide vinclozolin (VIN); or the vehicle, 6% dimethylsulfoxide in sesame oil (VEH). In adulthood, F1 male and female offspring were bred with untreated partners to generate paternal or maternal lineages of the F2 offspring, the subjects of molecular work. Quantitative real-time PCR was conducted in the medial preoptic area (POA) and the ventromedial nucleus (VMN) of the hypothalamus, selected for their roles in social and sexual behaviors. Of the genes assessed, steroid hormone receptors (estrogen receptor α, androgen receptor, progesterone receptor) but not dopamine receptors 1 and 2 or DNA methyltransferase 3a expression were altered, particularly in the VIN males. Several significant correlations between behavior and gene expression were also detected. These results suggest that preconceptional exposure of male rats to EDCs at the germ cell stage alters the neuromolecular phenotype in adulthood in a lineage-dependent manner.
PubMed ID: 31491443
MeSH Terms: Animals; Aroclors/pharmacology; Endocrine Disruptors/pharmacology*; Estrogen Receptor alpha/metabolism*; Female; Gene Expression Regulation/drug effects*; Hypothalamus/drug effects*; Hypothalamus/metabolism; Male; Oxazoles/pharmacology; Phenotype; Pregnancy; Prenatal Exposure Delayed Effects/metabolism*; Rats; Rats, Sprague-Dawley; Receptors, Androgen/metabolism*; Receptors, Progesterone/metabolism*