Title: High throughput fluorimetric assessment of iron traffic and chelation in iron-overloaded Caenorhabditis elegans.
Authors: Espósito, Breno Pannia; Martins, Airton Cunha; de Carvalho, Rodrigo Rodrigues Victor; Aschner, Michael
Published In Biometals, (2020 10)
Abstract: The nematode Caenorhabditis elegans (C. elegans) is a convenient tool to evaluate iron metabolism as it shares great orthology with human proteins involved in iron transport, in addition to being transparent and readily available. In this work, we describe how wild-type (N2) C. elegans nematodes in the first larval stage can be loaded with acetomethoxycalcein (CAL-AM) and study it as a whole-organism model for both iron speciation and chelator permeability of the labile iron pool (LIP). This model may be relevant for high throughput assessment of molecules intended for chelation therapy of iron overload diseases.
PubMed ID: 32979113
MeSH Terms: No MeSH terms associated with this publication