Title: BLM helicase complements disrupted type II telomere lengthening in telomerase-negative sgs1 yeast.
Authors: Lillard-Wetherell, Kate; Combs, Kelly A; Groden, Joanna
Published In Cancer Res, (2005 Jul 1)
Abstract: Recombination-mediated pathways for telomere lengthening may be utilized in the absence of telomerase activity. The RecQ-like helicases, BLM and Sgs1, are implicated in recombination-mediated telomere lengthening in human cells and budding yeast, respectively. Here, we show that BLM expression rescues disrupted telomere lengthening in telomerase-negative sgs1 yeast. BLM helicase activity is required for this complementation, indicating BLM and Sgs1 resolve the same telomeric structures. These data support a conserved function for BLM and Sgs1 in recombination-mediated telomere lengthening.
PubMed ID: 15994923
MeSH Terms: Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Adenosine Triphosphatases/physiology*; DNA Helicases/deficiency; DNA Helicases/genetics; DNA Helicases/metabolism; DNA Helicases/physiology*; Mutagenesis, Site-Directed; RecQ Helicases; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae Proteins/physiology*; Saccharomyces cerevisiae/enzymology*; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/ultrastructure; Telomerase/deficiency*; Telomere/genetics; Telomere/metabolism; Telomere/physiology*