Title: Development, validation and application of a chemiluminescent immunoassay for the measurement of circulating chorionic gonadotropin levels in the laboratory macaque.

Authors: Lohstroh, Pete; Laughlin, Lisa; Gee, Nancy; Lasley, Bill

Published In J Med Primatol, (2007 Jun)

Abstract: BACKGROUND: Methods for the measurement of chorionic gonadotropin for pregnancy detection in laboratory macaques chorionic gonadotropin (mCG) have been limited by the paucity of specific reagents. Current assays use reagents developed for human chorionic gonadotropin (hCG) assays and have been limited to radio- or enzyme-immuno assays. METHODS: This report describes an automated assay for mCG using reagents for hCG detection that has been adapted to the automated chemiluminescence platform of the Bayer ACS-180 autoanalyzer. RESULTS: This automated assay performs similarly to previous assays in terms of sensitivity and specificity but provides additional speed, reliability, and more easily accessible reagents compared with previous formats. CONCLUSIONS: When implemented broadly, this assay could provide standardization of mCG measurements for both research and colony management purposes.

PubMed ID: 17517091

MeSH Terms: Animals; Chemiluminescent Measurements/veterinary*; Chorionic Gonadotropin/blood*; Enzyme-Linked Immunosorbent Assay/veterinary; Female; Immunoassay/methods; Immunoassay/veterinary*; Macaca fascicularis/blood*; Macaca mulatta/blood*; Pregnancy; Pregnancy Tests/veterinary*; Sensitivity and Specificity