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National Institute of Environmental Health Sciences

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Publication Detail

Title: Optimized thiol derivatizing reagent for the mass spectral analysis of disubstituted epoxy fatty acids.

Authors: Newman, J W; Hammock, B D

Published In J Chromatogr A, (2001 Aug 03)

Abstract: A novel procedure is described for the derivatization of fatty acid epoxides in the presence of their corresponding diols. The acidic character of 2,3,5,6-tetrafluorobenzenethiol promotes favorable mass fragmentation of linoleate and arachidonate derived epoxide derivatives and reduces alkene isomerization to a manageable side reaction, eliminated through the addition of a thiol scavenger. After silylation, regioisomeric mixtures of epoxy- and dihydroxylipids are simultaneously detected and discriminated using gas chromatography with electron impact mass spectral detection. Silylated hydroxysulfanyloctadecanoids yielded instrumental detection limits of 5 pg/microl, sufficient sensitivity for the quantification of endogenous epoxylipids.

PubMed ID: 11519808 Exiting the NIEHS site

MeSH Terms: Epoxy Compounds/chemistry*; Fatty Acids/analysis*; Fatty Acids/chemistry; Gas Chromatography-Mass Spectrometry/methods*; Sulfhydryl Compounds/chemistry*

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