Title: Use of revertant cell lines to identify targets of v-fos transformation-specific alterations in gene expression.
Authors: Hoemann, C D; Zarbl, H
Published In Cell Growth Differ, (1990 Dec)
Abstract: Two proteins expressed in Rat-1 cells which are targets for v-fos transformation-specific alterations in gene expression were identified as alpha 1(I) and alpha 2(I) procollagen. While procollagen (I) proteins were synthesized in Rat-1 fibroblasts, their synthesis was dramatically reduced in Rat-1 cells transformed with the FBJ-v-fos oncogene. Revertant cell lines, which were previously shown to express a functional fos oncoprotein, resumed the synthesis of procollagen (I) at levels comparable to those seen in Rat-1 cells. Further results indicated that these procollagen proteins were also synthesized in Rat-1 cell lines that constitutively express high levels of a transfected c-fos protooncogene. Together, these observations suggested that constitutive fos protein expression was not sufficient to inhibit synthesis of these proteins. We have further demonstrated that Rat-1 cells transformed by most other oncogenes express abundant levels of procollagen (I), indicating that inhibition of procollagen (I) synthesis is not a general characteristic of transformed Rat-1 cells but is specifically associated with FBJ-v-fos-induced transformation. Northern blot analysis and runoff transcription assay data indicated that the alpha 1(I) procollagen, but not alpha 2(I) procollagen, is differentially regulated at the transcriptional level in Rat-1 fibroblasts, v-fos transformants, and revertants.
PubMed ID: 2126949
MeSH Terms: Amino Acid Sequence; Animals; Cell Line; Cell Line, Transformed; Cell Transformation, Neoplastic/genetics*; Gene Expression Regulation, Neoplastic; Glycosylation; Molecular Sequence Data; Molecular Weight; Oncogene Proteins v-fos; Oncogene Proteins, Viral/genetics*; Procollagen/biosynthesis*; Procollagen/genetics; RNA Processing, Post-Transcriptional; RNA, Messenger/biosynthesis; Rats; Transcription, Genetic