Superfund Research Program
Project Leader: Stephen H. Safe
Grant Number: P42ES004917
Funding Period: 1995 - 2000
Researchers developed a battery of E2-responsive bioassays for studies on AhR-mediated antiestrogenic activity and this objective has been expanded to investigate estrogenic activities of xenoestrogens and their mixtures. Research on endocrine disruptors was conducted in collaboration with scientists from the Chemical Industry Institute of Toxicology (Dr. Kevin Gaido), Duke University School of Medicine (Dr. Donald McDonnell), and NIEHS (Dr. Ken Korach). Because of this extensive inter-institutional collaboration, researchers were able to employ diverse in vivo and in vitro bioassays and our results demonstrated that interactions of weakly estrogenic pesticides and hydroxy-PCBs were essentially additive. Results of proposed studies will reduce the uncertainty in the risk assessment process for these Superfund chemicals. E2-responsive bioassays developed by these researchers were also used for environmental exposure assessment utilizing groundwater and soil extracts from areas in South Texas. The bioassays detected estrogenic activity in some extracts and the presence of industrial phenolics was subsequently confirmed by GC-MS analysis. Thus, research on endocrine disruptors and their interactions has addressed important environmental and public health issues on risk/exposure assessment, stimulated new basic research on the mechanisms of action of xenoestrogens and resulted in new collaborative studies on environmental distribution of industrial-derived endocrine-active compounds.