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Your Environment. Your Health.

Dartmouth College: Dataset Details, ID=GSE97036

Superfund Research Program

Arsenic and Innate Immunity in Human Lung

Project Leader: Bruce A. Stanton
Grant Number: P42ES007373
Funding Period: 2005-2021

Project-Specific Links

Title: Effects of arsenic and Pseudomonas aeruginosa infection on gene expression in human airway epithelial cells

Accession Number: GSE97036

Link to Dataset:

Repository: Gene Expression Omnibus (GEO)

Data Type(s): Gene Expression

Experiment Type(s): Expression profiling by high throughput sequencing

Organism(s): Homo sapiens

Summary: Purpose: To determine effects of arsenic on gene expression in polarized primary human bronchial epithelial (HBE) cells and impact on transcriptional response to Pseudomonas aeruginosa infection Methods: mRNA profiles of HBE cells from 6 donors exposed to 0, 5, 10 or 50 ug/L total arsenic +/- Pseudomonas aeruginosa (48 samples) were generated using Illumina sequencing, aligned in CLC Genomics workbench and analyzed for DE in EdgeR Findings: 20-30 million reads were mapped per sample and transcripts were identified that were significantly differentially expressed in response to arsenic and Pseudomonas aeruginosa

Publication(s) associated with this dataset:
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