Superfund Research Program
Arsenic and Innate Immunity in Human Lung
Project Leader: Bruce A. Stanton
Grant Number: P42ES007373
Funding Period: 2005-2021
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PublicationsTitle: Effects of arsenic and Pseudomonas aeruginosa infection on gene expression in human airway epithelial cells
Accession Number: GSE97036
Link to Dataset: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE97036
Repository: Gene Expression Omnibus (GEO)
Data Type(s): Gene Expression
Experiment Type(s): Expression profiling by high throughput sequencing
Organism(s): Homo sapiens
Summary: Purpose: To determine effects of arsenic on gene expression in polarized primary human bronchial epithelial (HBE) cells and impact on transcriptional response to Pseudomonas aeruginosa infection Methods: mRNA profiles of HBE cells from 6 donors exposed to 0, 5, 10 or 50 ug/L total arsenic +/- Pseudomonas aeruginosa (48 samples) were generated using Illumina sequencing, aligned in CLC Genomics workbench and analyzed for DE in EdgeR Findings: 20-30 million reads were mapped per sample and transcripts were identified that were significantly differentially expressed in response to arsenic and Pseudomonas aeruginosa
Publication(s) associated with this dataset:- Goodale BC, Rayack EJ, Stanton BA. 2017. Arsenic alters transcriptional responses to Pseudomonas aeruginosa infection and decreases antimicrobial defense of human airway epithelial cells. Toxicol Appl Pharmacol 331:154-163. doi:10.1016/j.taap.2017.06.010 PMID:28625800 PMCID:PMC5568502