Superfund Research Program
Duke University Superfund Research Center - Developmental Co-Exposures: Mechanisms, Outcomes, and Remediation
Center Director: Heather M. Stapleton
Grant Number: P42ES010356
Funding Period: 2000-2027
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Title: Global transcriptome changes in HepG2 cells exposed to copper
Accession Number: GSE9539
Link to Dataset: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE9539
Repository: Gene Expression Omnibus (GEO)
Data Type(s): Gene Expression
Experiment Type(s): Expression profiling by array
Organism(s): Homo sapiens
Summary: Our hypothesis is that copper modulates the activity of multiple intracellular signal transduction pathways to affect transcription. We have previously shown that copper activates transcription through both metal- and oxidative stress-responsive signal transduction pathways. Since the global molecular mechanisms underlying copper toxicity have not been well elucidated in humans, we have profiled transcriptome changes in HepG2 cells exposed to 100, 200, 400 and 600 uM copper for 4, 8, 12 and 24 hours using a human oligonucleotide microarray. Differentially expressed genes were identified, and integrated into biological and functional pathways through Gene Ontology analysis. Global gene expression profile was overlaid onto biomolecular interaction networks and signal transduction cascades using pathway mapping and interactome identification.
Publication(s) associated with this dataset:- Song MO, Li J, Freedman JH. 2009. Physiological and toxicological transcriptome changes in HepG2 cells exposed to copper. Physiol Genomics 38(3):386-401. doi:10.1152/physiolgenomics.00083.2009 PMID:19549813 PMCID:PMC3774564