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Duke University Superfund Research Center - Developmental Co-Exposures: Mechanisms, Outcomes, and Remediation
Center Director: Heather M. Stapleton
Grant Number: P42ES010356
Funding Period: 2000-2027
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Title: mRNA-seq of wild-type C. elegans exposed to rotenone
Accession Number: GSE195584
Link to Dataset: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE195584
Repository: Gene Expression Omnibus (GEO)
Data Type(s): Gene Expression
Experiment Type(s): Expression profiling by high throughput sequencing
Organism(s): Caenorhabditis elegans
Summary: To investigate the role of mitochondrial disruption on modulating conserved immunometabolic molecular pathways, we performed a whole transcriptome paired-end mRNA-seq analysis on C. elegans worms exposed to 0.5µM rotenone (a Complex I inhibitor) , or vehicle (0.125% dimethyl sulfoxide) . These results revealed 179 differentially expressed genes (134 up, 45 down) enriched for terms such as detoxification, energy metabolism, or pathogen defense. Whole transcriptome data revealed an association with the UPRmt and HIF-1 regulatory pathways.
Publication(s) associated with this dataset:- Mello DF, Bergemann CM, Fisher K, Chitrakar R, Bijwadia SR, Wang Y, Caldwell A, Baugh LR, Meyer JN. 2022. Rotenone modulates Caenorhabditis elegans immunometabolism and pathogen susceptibility. Front Immunol 13:840272. doi:10.3389/fimmu.2022.840272 PMID:35273616 PMCID:PMC8902048