Superfund Research Program
Toxicant Exposures in Rhode Island: Past, Present, and Future
Center Director: Robert H. Hurt
Grant Number: P42ES013660
Funding Period: 2005-2021
Title: Small RNA sequencing analysis of sperm RNA after control, and 3 different dose treatments of ethylene glycol monomethyl ether.
Accession Number: PRJNA492909
Link to Dataset: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA492909
Data Type(s): Epigenetic Data
Experiment Type(s): Raw sequence reads
Organism(s): Rattus norvegicus
Summary: This study was designed to identify molecular biomarkers in the sperm of rats treated with a known testicular toxicant. Here, we exposed rats to a model testicular toxicant, ethylene glycol monomethyl ether, that negatively affects a sperm precursor cell. We waited for these cells to become sperm, and then used RNA sequencing to look at the whole population of small RNAs. Changes in these small RNAs may be used to predict testicular toxicity in clinical or pharmaceutical testing settings. These RNAs may also be associated with paternal epigenetic inheritance of disease, or toxicity. In these instances, the father may pass non-genetically based health problems to offspring through sperm, or may have unintended health effects in offspring due to the father being exposures to environmental contaminants or pharmaceutical compounds.