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University of Iowa: Dataset Details, ID=GSE193578

Superfund Research Program

Airborne PCBs and their Metabolites: Risk Factors for Adverse Neurodevelopmental Outcomes in Adolescence

Project Leader: Hans-Joachim Lehmler
Co-Investigators: Jonathan A. Doorn, Hanna E. Stevens
Grant Number: P42ES013661
Funding Period: 2020-2025
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Title: Human Preadipocyte Response to PCB126

Accession Number: GSE193578

Link to Dataset:

Repository: Gene Expression Omnibus (GEO)

Data Type(s): Gene Expression

Experiment Type(s): Expression profiling by high throughput sequencing

Organism(s): Homo sapiens

Summary: Purpose: The goal of this study was to perform an expression transcript profile over a timecourse of human preadipocytes that were treated with PCB126; Methods: Immortal human preadipocytes (NPAD) were treated with 10 uM PCB126 for 9 hrs, 1 day, or 3 days. RNA was extracted with TriZol, DNase treated, and purified using a Qiagen column. RNAseq was used to assess gene expression. Bioinformatic analyses were used to determine differential gene expression between PCB126 treated and vehicle-treated at the same timepoint.

Publication(s) associated with this dataset:
  • Gourronc FA, Helm BK, Robertson LW, Chimenti MS, Joachim-Lehmler H, Ankrum J, Klingelhutz AJ. 2022. Transcriptome sequencing of 3,3',4,4',5-Pentachlorobiphenyl (PCB126)-treated human preadipocytes demonstrates progressive changes in pathways associated with inflammation and diabetes. Toxicol Vitro 83:105396. doi:10.1016/j.tiv.2022.105396 PMID:35618242 PMCID:PMC9423686
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