Superfund Research Program

Garry R. Buettner

University of Iowa
Free Radical Radiation Biology
College of Medicine, EMRB 68
Iowa City, Iowa 52242-1101
Phone: 319-335-6749
Fax: 319-335-9112
Email: garry-buettner@uiowa.edu

Projects

• University of Iowa: PCBs: Metabolism, Genotoxicity and Gene Expression in vivo (2006-2020)

Publications

2021

• Carter CS, Huang SC, Searby CC, Cassaidy B, Miller MJ, Grzesik WJ, Piorczynski TB, Pak TK, Walsh SA, Acevedo M, Zhang Q, Mapuskar KA, Milne GL, Hinton AO, Guo D, Weiss R, Bradberry K, Taylor EB, Rauckhorst AJ, Dick DW, Akurathi V, Falls-Hubert KC, Wagner BA, Carter WA, Wang K, Norris AW, Rahmouni K, Buettner GR, Hansen JM, Spitz DR, Abel ED, Sheffield VC. 2021. Response to: An alternative hypothesis for why exposure to static magnetic and electric fields treats type 2 diabetes. Am J Physiol Endocrinol Metab doi:10.1152/ajpendo.00119.2021 PMID:33843283
• Stolwijk J, Stefely JA, Veling MT, van't Erve TJ, Wagner BA, Raife TJ, Buettner GR. 2021. Red blood cells contain enzymatically active GPx4 whose abundance anticorrelates with hemolysis during blood bank storage. Redox Biol 46(9):doi:10.1016/j.redox.2021.102073 PMID:34298465 PMCID:PMC8322435

2020

• Carter CS, Huang SC, Searby CC, Cassaidy B, Miller MJ, Grzesik WJ, Piorczynski TB, Pak TK, Walsh SA, Acevedo M, Zhang Q, Mapuskar KA, Milne GL, Hinton AO, Guo D, Weiss R, Bradberry K, Taylor EB, Rauckhorst AJ, Dick DW, Akurathi V, Falls-Hubert KC, Wagner BA, Carter WA, Wang K, Norris AW, Rahmouni K, Buettner GR, Hansen JM, Spitz DR, Abel ED, Sheffield VC. 2020. Exposure to static magnetic and electric fields treats type 2 diabetes. Cell Metab 32:561-574. doi:10.1016/j.cmet.2020.09.012 PMID:33027675
• Stolwijk J, Falls-Hubert KC, Searby CC, Wagner BA, Buettner GR. 2020. Simultaneous detection of the enzyme activities of GPx1 and GPx4 guide optimization of selenium in cell biological experiments. Redox Biol 32:15. doi:10.1016/j.redox.2020.101518 PMID:32278283 PMCID:PMC7150527
• Stolwijk J, Garje R, Sieren JC, Buettner GR, Zakharia Y. 2020. Understanding the redox biology of selenium in the search of targeted cancer therapies. Antioxidants (Basel) 9:doi:10.3390/antiox9050420 PMID:32414091 PMCID:PMC7278812

2019

• Buranasudja V, Doskey CM, Gibson AR, Wagner BA, Du J, Gordon DJ, Koppenhafer S, Cullen JJ, Buettner GR. 2019. Pharmacologic ascorbate primes pancreatic cancer cells for death by rewiring cellular energetics and inducing DNA damage. Mol Cancer Res 17:2102-14. doi:10.1158/1541-7786.MCR-19-0381 PMID:31337671 PMCID:PMC6774825

2017

• Buettner GR, Buranasudja V, Doskey CM, Wagner BA. 2017. Estimating and partitioning the flux of ATP in cells in culture using mol cell(-1) s(-1) and the stoichiometries of metabolism. Free Radic Biol Med 112:161-161. doi:10.1016/j.freeradbiomed.2017.10.248
• Buranasudja V, Doskey CM, Wagner BA, Du J, Gordon DJ, Koppenhafer S, Cullen JJ, Buettner GR. 2017. DNA damage and energy crisis are central in the mechanism for the cytotoxicity of pharmacological ascorbate in cancer treatment. Free Radic Biol Med 112:162-162. doi:10.1016/j.freeradbiomed.2017.10.249

2016

• Anderson DG, Florang V, Schamp J, Buettner GR, Doorn JA. 2016. Antioxidant-mediated modulation of protein reactivity for 3,4-dihydroxyphenylacetaldehyde, a toxic dopamine metabolite. Chem Res Toxicol 29(7):1098-1107. doi:10.1021/acs.chemrestox.5b00528 PMID:27268734 PMCID:PMC5248556
• Doskey CM, Buranasudja V, Wagner BA, Wilkes JG, Du J, Cullen JJ, Buettner GR. 2016. Tumor cells have decreased ability to metabolize H2O2: Implications for pharmacological ascorbate in cancer therapy. Redox Biol 10:274-284. doi:10.1016/j.redox.2016.10.010 PMID:27833040 PMCID:PMC5106370
• Wangpradit O, Rahaman A, Mariappan S, Buettner GR, Robertson LW, Luthe G. 2016. Breaking the dogma: PCB-derived semiquinone free radicals do not form covalent adducts with DNA, GSH, and amino acids. Environ Sci Pollut Res Int 23(3):2138-2147. doi:10.1007/s11356-015-5007-4 PMID:26396011 PMCID:PMC4767158
• Xiao W, Sarsour EH, Wagner BA, Doskey CM, Buettner GR, Domann FE, Goswami PC. 2016. Succinate dehydrogenase activity regulates PCB3-quinone-induced metabolic oxidative stress and toxicity in HaCaT human keratinocytes. Arch Toxicol 90(2):319-332. doi:10.1007/s00204-014-1407-3 PMID:25417049 PMCID:PMC4441874

2015

• Cieslak JA, Strother RK, Rawal M, Du J, Doskey CM, Schroeder SR, Button AM, Wagner BA, Buettner GR, Cullen JJ. 2015. Manganoporphyrins and ascorbate enhance gemcitabine cytotoxicity in pancreatic cancer. Free Radic Biol Med 83:227-237. doi:10.1016/j.freeradbiomed.2015.02.018 PMID:25725418 PMCID:PMC4441864
• Doskey CM, van't Erve TJ, Wagner BA, Buettner GR. 2015. Moles of a substance per cell is a highly informative dosing metric in cell culture. PLoS One 10(7):e0132572. doi:10.1371/journal.pone.0132572 PMID:26172833 PMCID:PMC4501792
• van't Erve TJ, Wagner BA, Martin SM, Knudson C, Blendowski R, Keaton M, Holt T, Hess JR, Buettner GR, Ryckman KK, Darbro BW, Murray JC, Raife TJ. 2015. The heritability of hemolysis in stored human red blood cells. Transfusion 55(6):1178-1185. doi:10.1111/trf.12992 PMID:25644965 PMCID:PMC4469565
• Zhao H, Jiang J, Wang Y, Lehmler H, Buettner GR, Quan X, Chen J. 2015. Monohydroxylated polybrominated diphenyl ethers (OH-PBDEs) and dihydroxylated polybrominated biphenyls (Di-OH-PBBs): novel photoproducts of 2,6-dibromophenol. Environ Sci Technol 49(24):14120-14128. doi:10.1021/acs.est.5b03637 PMID:26545041 PMCID:PMC4717839

2014

• van't Erve TJ, Doskey CM, Wagner BA, Hess JR, Darbro BW, Ryckman KK, Murray JC, Raife TJ, Buettner GR. 2014. Heritability of glutathione and related metabolites in stored red blood cells. Free Radic Biol Med 76:107-113. doi:10.1016/j.freeradbiomed.2014.07.040 PMID:25108189 PMCID:PMC4252477
• van't Erve TJ, Wagner BA, Martin SM, Knudson C, Blendowski R, Keaton M, Holt T, Hess JR, Buettner GR, Ryckman KK, Darbro BW, Murray JC, Raife TJ. 2014. The heritability of metabolite concentrations in stored human red blood cells. Transfusion 54(8):2055-2063. doi:10.1111/trf.12605 PMID:24601981 PMCID:PMC4138246

2013

• Buettner GR, Wagner BA, Rodgers VG. 2013. Quantitative redox biology: an approach to understand the role of reactive species in defining the cellular redox environment. Cell Biochem Biophys 67(2):477-483. doi:10.1007/s12013-011-9320-3 PMID:22161621 PMCID:PMC3661692
• Olney KE, Du J, van't Erve TJ, Witmer JR, Sibenaller ZA, Wagner BA, Buettner GR, Cullen JJ. 2013. Inhibitors of hydroperoxide metabolism enhance ascorbate-induced cytotoxicity. Free Radic Res 47(3):154-163. doi:10.3109/10715762.2012.755263 PMID:23205739 PMCID:PMC3674878
• Rawal M, Schroeder SR, Wagner BA, Cushing CM, Welsh JL, Button AM, Du J, Sibenaller ZA, Buettner GR, Cullen JJ. 2013. Manganoporphyrins increase ascorbate-induced cytotoxicity by enhancing H₂O₂ generation. Cancer Res 73(16):5232-5241. doi:10.1158/0008-5472.can-13-0470 PMID:23764544 PMCID:PMC3745518
• van't Erve TJ, Wagner BA, Ryckman KK, Raife TJ, Buettner GR. 2013. The concentration of glutathione in human erythrocytes is a heritable trait. Free Radic Biol Med 65:742-749. doi:10.1016/j.freeradbiomed.2013.08.002 PMID:23938402 PMCID:PMC3859832
• Wagner BA, Witmar JR, van't Erve TJ, Buettner GR. 2013. An assay for the rate of removal of extracellular hydrogen peroxide by cells. Redox Biol 1:210-217. doi:10.1016/j.redox.2013.01.011 PMID:23936757 PMCID:PMC3736862
• Welsh JL, Wagner BA, van't Erve TJ, Zehr PS, Berg DJ, Halfdanarson TR, Yee NS, Bodeker KL, Du J, Roberts LJ, Drisko J, Levine M, Buettner GR, Cullen JJ. 2013. Pharmacological ascorbate with gemcitabine for the control of metastatic and node-positive pancreatic cancer (PACMAN): results from a phase I clinical trial. Cancer Chemother Pharmacol 71(3):765-775. doi:10.1007/s00280-013-2070-8 PMID:23381814 PMCID:PMC3587047

2012

• Sarsour EH, Kalen AL, Xiao Z, Veenstra TD, Chaudhuri L, Venkataraman S, Reigan P, Buettner GR, Goswami PC. 2012. Manganese superoxide dismutase regulates a metabolic switch during the Mammalian Cell Cycle. Cancer Res 72:3807-3816. doi:10.1158/0008-5472.CAN-11-1063 PMID:22710435 PMCID:PMC3429130

2011

• Anderson DG, Mariappan S, Buettner GR, Doorn JA. 2011. Oxidation of 3,4-dihydroxyphenylacetaldehyde, a toxic dopaminergic metabolite, to a semiquinone radical and an ortho-quinone. J Biol Chem 286:26978-26986. doi:10.1074/jbc.M111.249532 PMID:21642436 PMCID:PMC3143656
• Buettner GR. 2011. Superoxide dismutase in redox biology: the roles of superoxide and hydrogen peroxide. Anticancer Agents Med Chem 11(4):341-346. PMID:21453242 PMCID:PMC3131414
• Sarsour EH, Kumar MG, Kalen AL, Goswami M, Buettner GR, Goswami PC. 2011. MnSOD activity regulates hydroxytyrosol-induced extension of chronological lifespan. Age (Dordr) 34(1):95-109. doi:10.1007/s11357-011-9223-7 PMID:21384152 PMCID:PMC3260369
• Wagner BA, Venkataraman S, Buettner GR. 2011. The rate of oxygen utilization by cells. Free Radic Biol Med 51(3):700-712. doi:10.1016/j.freeradbiomed.2011.05.024 PMID:21664270 PMCID:PMC3147247

2010

• Eslami A, Pasanphan W, Wagner BA, Buettner GR. 2010. Free radicals produced by the oxidation of gallic acid: an electron paramagnetic resonance study. Chem Cent J 4:15. doi:10.1186/1752-153x-4-15 PMID:20687941 PMCID:PMC2924338
• Song Y, Buettner GR. 2010. Thermodynamic and kinetic considerations for the reaction of semiquinone radicals to form superoxide and hydrogen peroxide. Free Radic Biol Med 49:919-962. doi:10.1016/j.freeradbiomed.2010.05.009 PMID:20493944 PMCID:PMC2936108
• Wangpradit O, Moman E, Nolan K, Buettner GR, Robertson LW, Luthe G. 2010. Observation of an unusual electronically distorted semiquinone radical of PCB metabolites in the active site of prostaglandin H synthase-2. Chemosphere 81:1501-1508. doi:10.1016/j.chemosphere.2010.08.041 PMID:20843536 PMCID:PMC3622869

2009

• Song Y, Wagner BA, Witmer JR, Lehmler H, Buettner GR. 2009. Nonenzymatic displacement of chlorine and formation of free radicals upon the reaction of glutathione with PCB quinones. Proc Natl Acad Sci U S A 106(24):9725-9730. doi:10.1073/pnas.0810352106 PMID:19497881 PMCID:PMC2689812
• Yang-won S, Buettner GR, Venkataraman S, Treimer S, Robertson LW, Ludewig G. 2009. UVA/B-induced formation of free radicals from decabromodiphenyl ether. Environ Sci Technol 43(7):2581-2588. PMID:19452920 PMCID:PMC2685467

2008

• Song Y, Buettner GR, Parkin S, Wagner BA, Robertson LW, Lehmler H. 2008. Chlorination increases the persistence of semiquinone free radicals derived from polychlorinated biphenyl hydroquinones and quinones. J Org Chem 73(21):8296-8304. doi:10.1021/jo801397g PMID:18839991 PMCID:PMC2629131
• Venkatesha VA, Venkataraman S, Sarsour EH, Kalen AL, Buettner GR, Robertson LW, Lehmler H, Goswami PC. 2008. Catalase ameliorates polychlorinated biphenyl-induced cytotoxicity in nonmalignant human breast epithelial cells. Free Radic Biol Med 45(8):1094-1102. doi:10.1016/j.freeradbiomed.2008.07.007 PMID:18691649 PMCID:PMC2614346