Superfund Research Program

Natalia Vasylieva

University of California-Davis
One Shields Ave
Davis, California 95616-5270
Phone: 530-752-7519
Email: nvasylieva@ucdavis.edu

Projects

• University of California-Davis: Immunoassays for Human and Environmental Health Monitoring (1995-2023)

Patents

• Recombinant nanobodies (VHHs) deactivating Pseudomonas aeruginosa virulence factor Cif – tools for drug development, application in diagnostics and clinical treatment

Publications

2023

• Yang H, Vasylieva N, Wang J, Li Z, Duan W, Chen S, Wen K, Meng H, Yu X, Shen J, Hammock BD, Wang Z. 2023. Precise isolation and structural origin of an ultra-specific nanobody against chemical compound. J Hazard Mater 458:131958. doi:10.1016/j.jhazmat.2023.131958 PMID:37429192

2022

• Salvador J, Vasylieva N, Gonzalez-Garcia I, Jin M, Caster RW, Siegel JB, Hammock BD. 2022. Nanobody-based lateral flow immunoassay for the rapid detection of aflatoxin B1 in almond milk. ACS Food Sci Technol 2(8):1276-1282. doi:10.1021/acsfoodscitech.2c00118

2021

• Wang F, Li Z, Wan D, Vasylieva N, Shen Y, Xu Z, Yang J, Gettemans J, Wang H, Hammock BD, Sun Y. 2021. Enhanced non-toxic immunodetection of Alternaria mycotoxin tenuazonic acid based on ferritin-displayed anti-idiotypic nanobody-nanoluciferase multimers. J Agric Food Chem 69:4911-4917. doi:10.1021/acs.jafc.1c01128 PMID:33870684
• Xu B, Wang K, Vasylieva N, Zhou H, Xue X, Wang B, Li QX, Hammock BD, Xu T. 2021. Development of a nanobody-based ELISA for the detection of the insecticides cyantraniliprole and chlorantraniliprole in soil and the vegetable bok choy. Anal Bioanal Chem 413(9):2503-2511. doi:10.1007/s00216-021-03205-x PMID:33580830
• Zhao Y, Ruan X, Song Y, Smith JN, Vasylieva N, Hammock BD, Lin Y, Du D. 2021. Smartphone-based dual-channel immunochromatographic test strip with polymer quantum dot labels for simultaneous detection of cypermethrin and 3-phenoxybenzoic acid. Anal Chem 93:PMID:34591463
• Zhao Y, Wang M, Zhang M, Yang X, Li Z, Vasylieva N, Tan G, Wang B, Hammock BD. 2021. Development of a direct competitive enzyme-linked immunosorbent assay for quantitation of sodium saccharin residue in food. J Food Sci 10:doi:10.1111/1750-3841.15767 PMID:34309034

2020

• El-Moghazy AY, Huo J, Amaly N, Vasylieva N, Hammock BD, Sun G. 2020. An innovative nanobody-based electrochemical immunosensor using decorated nylon nanofibers for point-of-care monitoring of human exposure to pyrethroid insecticides. ACS Appl Mater Interfaces 12:6159-6168. doi:10.1021/acsami.9b16193
• Kumar S, Vasylieva N, Singh V, Hammock BD, Singh SG. 2020. A facile, sensitive and rapid sensing platform based on CoZnO for detection of fipronil; an environmental toxin. Electroanalysis 10:doi:10.1002/elan.202000051
• Li P, Yang C, Liu B, Wu Q, Wang Y, Dong S, Zhang H, Vasylieva N, Hammock BD, Zhang C. 2020. Sensitive immunochromatographic assay using highly luminescent quantum dot nanobeads as tracer for the detection of cyproheptadine hydrochloride in animal-derived food. Front Chem 8:575. doi:10.3389/fchem.2020.00575 PMID:32760698 PMCID:PMC7372008
• Li Z, Dong J, Vasylieva N, Cui Y, Wan D, Hua X, Huo J, Yang D, Gee SJ, Hammock BD. 2020. Highly specific nanobody against herbicide 2,4-dichlorophenoxyacetic acid for monitoring of its contamination in environmental water. Sci Total Environ 753:doi:10.1016/j.scitotenv.2020.141950 PMID:32906044 PMCID:PMC7674261
• Li Z, Wang Y, Vasylieva N, Wan D, Yin Z, Dong J, Hammock BD. 2020. An ultrasensitive bioluminescent enzyme immunoassay based on nanobody/nanoluciferase heptamer fusion for the detection of Tetrabromobisphenol A in sediment. Anal Chem 92:10083-10090. doi:10.1021/acs.analchem.0c01908 PMID:32559059
• Qi M, Huo J, Li Z, He C, Li D, Wang Y, Vasylieva N, Zhang J, Hammock BD. 2020. On-spot quantitative analysis of dicamba in field waters using a lateral flow immunochromatographic strip with smartphone imaging. Anal Bioanal Chem doi:10.1007/s00216-020-02833-z PMID:32737550
• Wang F, Li Z, Yang Y, Wan D, Vasylieva N, Zhang Y, Cai J, Wang H, Shen Y, Xu Z, Hammock BD. 2020. Chemiluminescent enzyme immunoassay and bioluminescent enzyme immunoassay for tenuazonic acid mycotoxin by exploitation of nanobody and nanobody-nanoluciferase fusion. Anal Chem 92:11935-11942. doi:10.1021/acs.analchem.0c02338 PMID:32702970

2019

• Huo J, Barnych B, Li Z, Wan D, Li D, Vasylieva N, Knezevic SZ, Osipitan OA, Scott JE, Zhang J, Hammock BD. 2019. Hapten synthesis, antibody development, and a highly sensitive indirect competitive chemiluminescent enzyme immunoassay for detection of dicamba. J Agric Food Chem 67:5711-5719. doi:10.1021/acs.jafc.8b07134 PMID:31042038 PMCID:PMC6873229
• Joshi U, Pearson A, Evans JE, Langlois H, Saltiel N, Ojo J, Klimas N, Sullivan K, Keegan AP, Oberlin S, Darcey T, Cseresznye A, Raya B, Paris D, Hammock BD, Vasylieva N, Hongsibsong S, Stern LJ, Crawford F, Mullan M, Abdullah L. 2019. A permethrin metabolite is associated with adaptive immune responses in Gulf War Illness. Brain Behav Immun 81:545-559. doi:10.1016/j.bbi.2019.07.015
• Tang Z, Mo F, Liu A, Siliang D, Xiaomei Y, Liang L, Hou X, Yin S, Jiang X, Vasylieva N, Dong J, Barnych B, Hammock BD, Lu X. 2019. A nanobody against cytotoxic t-lymphocyte associated antigen-4 increases the anti-tumor effects of specific CD8(+) T cells. J Biomed Nanotechnol 15:2229-2239. doi:10.1166/jbn.2019.2859 PMID:31847937
• Vasylieva N, Kitamura S, Dong J, Barnych B, Hvorecny KL, Madden DR, Gee SJ, Wolan DW, Morisseau C, Hammock BD. 2019. Nanobody-based binding assay for the discovery of potent inhibitors of CFTR inhibitory factor (Cif). Anal Chim Acta 1057:106-113. doi:10.1016/j.aca.2018.12.060 PMID:30832908 PMCID:PMC6426448
• Wang K, Liu Z, Ding G, Li J, Vasylieva N, Li QX, Li D, Gee SJ, Hammock BD, Xu T. 2019. Development of a one-step immunoassay for triazophos using camel single-domain antibody-alkaline phosphatase fusion protein. Anal Bioanal Chem 411(6):1287-1295. doi:10.1007/s00216-018-01563-7
• Wang K, Vasylieva N, Wan D, Eads DA, Yang J, Tretten T, Barnych B, Li J, Li QX, Gee SJ, Hammock BD, Xu T. 2019. Quantitative detection of fipronil and fipronil-sulfone in sera of black-tailed prairie dogs and rats after oral exposure to fipronil by camel single-domain antibody-based immunoassays. Anal Chem 91:1532-1540. doi:10.1021/acs.analchem.8b04653
• Wang Y, Li Z, Barnych B, Huo J, Wan D, Vasylieva N, Xu J, Li P, Liu B, Zhang C, Hammock BD. 2019. Investigation of the small size of nanobodies for a sensitive fluorescence polarization immunoassay for small molecules: 3-phenoxybenzoic acid, an exposure biomarker of pyrethroid insecticides as a model. J Agric Food Chem 67(41):11536-11541. doi:10.1021/acs.jafc.9b04621

2018

• Cam M, Durieu E, Bodin M, Manousopoulou A, Koslowski S, Vasylieva N, Barnych B, Hammock BD, Bohl B, Koch P, Omori C, Yamamoto K, Hata S, Suzuki T, Karg F, Gizzi P, Haber V, Mihaljevic VB, Tavcar B, Portelius E, Pannee J, Blennow K, Zetterberg H, Garbis SD, Auvray P, Gerber H, Fraering J, Fraering PC, Meijer L. 2018. Induction of amyloid-beta(42) production by fipronil and other pyrazole insecticides. Journal of Alzheimers Disease 62:1663-1681. doi:10.3233/jad-170875 PMID:29504531
• Chen H, Yang Q, Ding Y, Vasylieva N, Bever C, Hua X, Wang M, Hammock BD. 2018. Competitive and noncompetitive immunoassays for the detection of benzothiostrobin using magnetic nanoparticles and fluorescein isothiocyanate-labeled peptides. Anal Bioanal Chem 1-99. doi:10.1007/s00216-018-1478-8 PMID:30478514
• Huo J, Li Z, Wan D, Li D, Qi M, Barnych B, Vasylieva N, Zhang J, Hammock BD. 2018. Development of a highly sensitive direct competitive fluorescence enzyme immunoassay based on a nanobody-alkaline phosphatase fusion protein for detection of 3-phenoxybenzoic acid in urine. J Agric Food Chem 66:11284-11290. doi:10.1021/acs.jafc.8b04521 PMID:30293433
• Pali M, Bever C, Vasylieva N, Hammock BD, Suni II. 2018. Impedance detection of 3-phenoxybenzoic acid with a noncompetitive two-site phage anti-immunocomplex assay. Electroanalysis 30:2653-2659. doi:10.1002/elan.201800457

2017

• Cui X, Vasylieva N, Wu P, Barnych B, Yang J, Shen D, He Q, Gee SJ, Zhao S, Hammock BD. 2017. Development of an indirect competitive enzyme-linked immunosorbent assay for glycocholic acid based on chicken single-chain variable fragment antibodies. Anal Chem 89:11091-11097. doi:10.1021/acs.analchem.7b03190 PMID:28942640
• Vasylieva N, Barnych B, Rand A, Inceoglu B, Gee SJ, Hammock BD. 2017. Sensitive immunoassay for detection and quantification of the neurotoxin, tetramethylenedisulfotetramine. Anal Chem 89(10):5612-5619. doi:10.1021/acs.analchem.7b00846 PMID:28398746
• Vasylieva N, Barnych B, Wan D, El-Sheikh EA, Nguyen HM, Wulff H, McMahen R, Strynar M, Gee SJ, Hammock BD. 2017. Hydroxy-fipronil is a new urinary biomarker of exposure to fipronil. Environ Int 103:91-98. doi:10.1016/j.envint.2017.03.012 PMID:28343720 PMCID:PMC5432128
• Wagner SD, Kurobe T, Hammock BG, Lam CH, Wu G, Vasylieva N, Gee SJ, Hammock BD, Teh SJ. 2017. Developmental effects of fipronil on Japanese Medaka (Oryzias latipes) embryos. Chemosphere 166:511-520. doi:10.1016/j.chemosphere.2016.09.069 PMID:27710886

2016

• Bever C, Dong J, Vasylieva N, Barnych B, Cui Y, Hammock BD, Gee SJ. 2016. VHH antibodies: emerging reagents for the analysis of environmental chemicals. Anal Bioanal Chem 408(22):5985-6002. doi:10.1007/s00216-016-9585-x PMID:27209591 PMCID:PMC4983233

2015

• Vasylieva N, Ahn K, Barnych B, Gee SJ, Hammock BD. 2015. Development of an immunoassay for the detection of the phenylpyrazole insecticide fipronil. Environ Sci Technol 49(16):10038-10047. doi:10.1021/acs.est.5b01005 PMID:26196357 PMCID:PMC4605820