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Person Details: Ronald H. Olsen

Superfund Research Program

Ronald H. Olsen

University of Michigan
Ann Arbor, Michigan




  • Kahng H, Byrne AM, Olsen RH, Kukor JJ. 2000. Characterization and role of tbuX in utilization of toluene by Ralstonia pickettii PKO1. J Bacteriol 182(5):1232-1242. PMID:10671442 PMCID:PMC94407


  • Johnson GR, Olsen RH. 1997. Multiple pathways for toluene degradation in Burkholderia sp. strain JS150. Appl Environ Microbiol 63(10):4047-4052. PMID:9327568
  • Leahy JG, Olsen RH. 1997. Kinetics of toluene degradation by toluene-oxidizing bacteria as a function of oxygen concentration and the effect of nitrate. FEMS Microbiol Lett 23:23-30.
  • Leahy JG, Olsen RH, Johnson GR. 1997. Cross-regulation of toluene monooxygenases by the transcriptional activators TbmR and TbuT. Appl Environ Microbiol 63(9):3736-3739. PMID:9293027
  • Olsen RH, Kukor JJ, Byrne AM, Johnson GR. 1997. Evidence for the evolution of a single component phenol/cresol hydroxylase from a multicomponent toluene monooxygenase. J Ind Microbiol Biotechnol 19(5-6):360-368. PMID:9451833
  • Olsen RH, Kukor JJ, Byrne AM, Johnson GR. 1997. Recruitment of an alkyl-substituted phenol/benzene regulated system for the evolution of a unit peptide phenol hydroxylase. In: Proceedings of the VIth International Congress on Pseudomonas. pp.133.


  • Byrne AM, Olsen RH. 1996. Cascade regulation of the toluene-3-monooxygenase operon (tbuA1UBVA2C) of Burkholderia pickettii PKO1: role of the tbuA1 promoter (PtbuA1) in the expression of its cognate activator, TbuT. J Bacteriol 178(21):6327-6337. PMID:8892837 PMCID:PMC178508
  • Byrne AM, Olsen RH. 1996. Nucleotide sequence analysis of tbuX, a gene encoding a putative membrane-associated protein from Burkholderia pickettii PKO1. Bacteriology Procedures
  • Kukor JJ, Olsen RH. 1996. Catechol 2,3-dioxygenases functional in oxygen-limited (hypoxic) environments. Appl Environ Microbiol 62(5):1728-1740. PMID:8633871 PMCID:PMC167947
  • Leahy JG, Byrne AM, Olsen RH. 1996. Comparison of factors influencing trichloroethylene degradation by toluene-oxidizing bacteria. Appl Environ Microbiol 62(3):825-833. PMID:8975612
  • Olsen RH, Kukor JJ, Leahy JG, Johnson GR. 1996. "Natural" genetic engineering for the metabolism of trichloroethylene and xylene hydrocarbons in low-oxygen environments. In: Proceedings of the 35th Hanford Symposium on Health and the Environment, Microbial Genome Research and its Applications. Richland, WA. pp.55-56.


  • Byrne AM, Kukor JJ, Olsen RH. 1995. Sequence analysis of the gene cluster encoding toluene-3-monooxygenase from Pseudomonas pickettii PKO1. Gene 154(1):65-70. PMID:7867951
  • Johnson GR, Olsen RH. 1995. Nucleotide sequence analysis of genes encoding a toluene/benzene-2-monooxygenase from Pseudomonas sp strain JS150. Appl Environ Microbiol 61(9):3336-3346. PMID:7574644 PMCID:PMC167614
  • Olsen RH, Mikesell MD, Kukor JJ, Byrne AM. 1995. Physiological attributes of microbial BTEX degradation in oxygen-limited environments. Environ Health Perspect 103(supp.5):49-51. PMID:8565910


  • Kukor JJ, Olsen RH. 1992. Complete nucleotide sequence of tbuD, the gene encoding phenol/cresol hydroxylase from Pseudomonas pickettii PKO1, and functional analysis of the encoded enzyme. J Bacteriol 174(20):6518-6526. PMID:1400204 PMCID:PMC207615
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