Superfund Research Program

Robert C. Burghardt

Texas A&M University
Veterinary Integrative Biosciences
B12A VMA
4458 TAMU
College Station, Texas 77843-4458
Phone: 979-862-4083
Fax: 979-847-8981
Email: rburghardt@cvm.tamu.edu

Projects

• Texas A&M University: Image Analysis and Bioassays Core (2000-2008)

Publications

2014

• Barhoumi R, Mouneimne Y, Chapkin RS, Burghardt RC. 2014. Effects of fatty acids on benzo[a]pyrene uptake and metabolism in human lung adenocarcinoma A549 cells. PLoS One 9(3):e90908. doi:10.1371/journal.pone.0090908 PMID:24651647 PMCID:PMC3961214

2011

• Barhoumi R, Mouneimne Y, Hammock BD, Safe SH, Burghardt RC, Parrish AR, Ramos E. 2011. Multiphoton spectral analysis of benzo a pyrene uptake and metabolism in a rat liver cell line. Toxicol Appl Pharmacol 253:45-56. doi:10.1016/j.taap.2011.03.009 PMID:21420996 PMCID:PMC3081912
• Kim K, Burghardt RC, Lee S, Liu X, Safe SH, Barhoumi R. 2011. MDM2 regulates estrogen receptor alpha and estrogen responsiveness in breast cancer cells. J Mol Endocrinol 46:67-79. doi:10.1677/jme-10-0110 PMID:21169420 PMCID:PMC3069690

2010

• Kim J, Erikson D, Burghardt RC, Spencer T, Wu G, Bayless K, Johnson G, Bazer F. 2010. Secreted phosphoprotein 1 binds integrins to initiate multiple cell signaling pathways, including FRAP1/mTOR, to support attachment and force-generated migration of trophectoderm cells. Matrix Biology 29:369-382. doi:10.1016/j.matbio.2010.04.001 PMID:20385232

2009

• Barhoumi R, Catania JM, Parrish AR, Awooda I, Tiffany-Castiglioni E, Safe SH, Burghardt RC. 2009. Multiphoton spectral analysis of benzo[a]pyrene uptake and metabolism in breast epithelial cell lines. J Toxicol Sci 34(1):13-25. doi:10.2131/jts.34.13 PMID:19182432 PMCID:PMC2739812
• Martinez JG, Huang J, Burghardt RC, Barhoumi R, Carroll RJ. 2009. Use of multiple singular value decompositions to analyze complex intracellular calcium ion signals. Annals of Applied Statistics 3(4):1467-1492. doi:10.1214/09-aoas253 PMID:20428493 PMCID:PMC2859732
• Parrish AR, Chen G, Burghardt RC, Watanabe T, Hammock BD, Morisseau C. 2009. Attenuation of cisplatin nephrotoxicity by inhibition of soluble epoxide hydrolase. Cell Biol Toxicol 25(3):217-225. doi:10.1007/s10565-008-9071-0 PMID:18386137 PMCID:PMC3033346

2008

• Wu F, Khan S, Wu Q, Barhoumi R, Burghardt RC, Safe SH. 2008. Ligand structure-dependent activation of estrogen receptor alpha/Sp by estrogens and xenoestrogens. Psychiatr Pol 110(1-2):104-115. doi:10.1016/j.jsbmb.2008.02.008 PMID:18400491 PMCID:PMC2519242

2006

• Abdelrahim M, Ariazi E, Kim K, Khan S, Barhoumi R, Burghardt RC, Liu S, Hill DS, Finnell RH, Wlodarczyk BJ, Jordan VC, Safe SH. 2006. 3-Methylcholanthrene and other aryl hydrocarbon receptor agonists directly activate estrogen receptor alpha. Cancer Res 66(4):2459-2467. doi:10.1158/0008-5472.CAN-05-3132 PMID:16489053
• Barhoumi R, Awooda I, Mouneimne Y, Safe SH, Burghardt RC. 2006. Effects of benzo-a-pyrene on oxytocin-induced Ca2+ oscillations in myometrial cells. Toxicol Lett 165(2):133-141. PMID:16567066

2005

• Ottinger SE, Barhoumi R, McKenzie KS, McDonald TJ, Burghardt RC, Huebner HJ, Phillips TD. 2005. FIA/MS analysis of temporally ozonated benzo[a]pyrene and pyrene and their reaction products: inhibition of gap junction-mediated intercellular communication. Chemosphere 60(8):1025-1033. doi:10.1016/j.chemosphere.2005.01.025 PMID:15993149

2004

• Herrera P, Burghardt RC, Huebner HJ, Phillips TD. 2004. The efficacy of sand-immobilized organoclays as filtration bed materials for bacteria. Food Microbiol 21(1):1-10.
• Jiang J, Dean D, Burghardt RC, Parrish AR. 2004. Disruption of cadherin/catenin expression, localization, and interactions during HgCl2-induced nephrotoxicity. Toxicol Sci 80(1):170-182. PMID:15084754

2003

• Cizmas LH, Barhoumi R, Burghardt RC, Reeves WR, He L, McDonald TJ, Donnelly KC. 2003. A comparison of two methods for fractionating complex mixtures in preparation for toxicity analysis. J Toxicol Environ Health A 66(14):1351-1370. PMID:12851116
• Hong MS, Hong SJ, Barhoumi R, Burghardt RC, Donnelly KC, Wild JR, Venkatraj V, Tiffany-Castiglioni E. 2003. Neurotoxicity induced in differentiated SK-N-SH-SY5Y human neuroblastoma cells by organophosphorus compounds. Toxicol Appl Pharmacol 186(2):110-118. PMID:12639502
• Qin C, Burghardt RC, Smith RL, Wormke M, Stewart J, Safe SH. 2003. Peroxisome proliferator-activated receptor gamma agonists induce proteasome-dependent degradation of cyclin D1 and estrogen receptor alpha in MCF-7 breast cancer cells. Cancer Res 63(5):958-964. PMID:12615709
• Wormke M, Stoner M, Saville B, Walker K, Abdelrahim M, Burghardt RC, Safe SH. 2003. The aryl hydrocarbon receptor mediates degradation of estrogen receptor alpha through activation of proteasomes. Mol Cell Biol 23(6):1842-1855. PMID:12612060

2002

• Abdelrahim M, Samudio I, Smith, III R, Burghardt RC, Safe SH. 2002. Small inhibitory RNA duplexes for Sp1 mRNA block basal and estrogen-induced gene expression and cell cycle progression in MCF-7 breast cancer cells. J Biol Chem 277(32):28815-28822. PMID:12052832
• Barhoumi R, Mouneimne Y, Awooda I, Safe SH, Donnelly KC, Burghardt RC. 2002. Characterization of calcium oscillations in normal and benzo[a]pyrene-treated clone 9 cells. Toxicol Sci 68(2):444-450. PMID:12151640
• Mealey KL, Barhoumi R, Burghardt RC, Safe SH, Kochevar DT. 2002. Doxycycline induces expression of P glycoprotein in MCF-7 breast carcinoma cells. Antimicrobial Agents and Chemotherapy 46(3):755-761. PMID:11850258
• Qin C, Nguyen T, Stewart J, Burghardt RC, Safe SH. 2002. Estrogen up-regulation of p53 gene expression in MCF-7 breast cancer cells is mediated, by calmodulin kinase IV-dependent activation of a nuclear factor kappa B/CCAAT-binding transcription factor-1 complex. Mol Endocrinol 16(8):1793-1809. PMID:12145335
• Stoner M, Saville B, Wormke M, Dean D, Burghardt RC, Safe SH. 2002. Hypoxia induces proteasome-dependent degradation of estrogen receptor a in ZR-75 breast cancer cells. Mol Endocrinol 16(10):2231-2242. PMID:12351689

2001

• Duan R, Xie W, Burghardt RC, Safe SH. 2001. Estrogen receptor-mediated activation of the serum response element in MCF-7 cells through MAPK-dependent phosphorylation of Elk-1. J Biol Chem 276(15):11590-11598. doi:10.1074/jbc.M005492200 PMID:11145955
• Reeves WR, Barhoumi R, Burghardt RC, Lemke SL, Mayura K, McDonald TJ, Phillips TD, Donnelly KC. 2001. Evaluation of methods for predicting the toxicity of polycyclic aromatic hydrocarbon mixtures. Environ Sci Technol 35(8):1630-1636. doi:10.1021/es001689a PMID:11329713
• Samudio I, Vyhlidal C, Wang F, Stoner M, Chen I, Kiadde M, Barhoumi R, Burghardt RC, Safe SH. 2001. Transcriptional activation of deoxyribonucleic acid polymerase alpha gene expression in MCF-7 cells by 17 beta-estradiol. Endocrinology 142(3):1000-1008. PMID:11181512
• Washburn KS, Donnelly KC, Huebner HJ, Burghardt RC, Sewall TC, Claxton LD. 2001. A study of 2,4,6-trinitrotoluene inhibition of benzo[a]pyrene uptake and activation in a microbial mutagenicity assay. Chemosphere 44(8):1703-1709. PMID:11534902

2000

• Alejandro NF, Parrish AR, Bowes RC, Burghardt RC, Ramos KS. 2000. Phenotypic profiles of cultured glomerular cells following repeated cycles of hydrocarbon injury. Kidney International 57:1571-1580. PMID:10760092
• Barhoumi R, Mouneimne Y, Ramos KS, Safe SH, Phillips TD, Centonze VE, Ainley C, Gupta MS, Burghardt RC. 2000. Analysis of Benzo[a]pyrene partitioning and cellular homeostasis in a rat liver cell line. Toxicol Sci 53:264-270. PMID:10696774
• Herrera P, Burghardt RC, Phillips TD. 2000. Adsorption of Salmonella enteritidis by cetyl pyridinium exchanged montmorillonite clays. Vet Microbiol 74:259-272. PMID:10808094
• Legare ME, Hanneman WH, Barhoumi R, Burghardt RC, Tiffany-Castiglioni E. 2000. 2,3,7,8-tetrachlorodibenzo-p-dioxin alters hippocampal astroglia-neuronal gap junctional communication. Neurotoxicology 21(6):1109-1116. PMID:11233757

1998

• Burghardt RC, Barhoumi R, Sanborn BM, Andersen J. 1998. Oxytocin-induced Ca²⁺ responses in human myometrial cells. Biol Reprod 60:777-782. PMID:10084948
• Hutchinson RW, Barhoumi R, Miles J, Burghardt RC. 1998. Attenuation of gossypol cytotoxicity by cyclic AMP in a rat liver cell line. Toxicol Appl Pharmacol 151:311-318. PMID:9707507
• Hutchinson RW, Ing NH, Burghardt RC. 1998. Induction of c-fos and cytochrome C oxidase subunits I and II by gossypol acetic acid in rat liver cells. Cell Biol Toxicol 14(6):391-399. PMID:9879931
• Legare ME, Barhoumi R, Hebert E, Bratton GR, Burghardt RC, Tiffany-Castiglioni E. 1998. Analysis of Pb2+ entry into cultured astroglia. Toxicol Sci 46(1):90-100. doi:10.1006/toxs.1998.2492 PMID:9928672
• Parrish AR, Fisher RL, Bral CM, Burghardt RC, Gandolfi A, Brendel K, Ramos KS. 1998. Benzo(a)pyrene-induced alterations in growth-related gene expression and signaling in precision-cut adult rat liver slices. Toxicol Appl Pharmacol 152:302-308. PMID:9852999
• Thompson DC, Barhoumi R, Burghardt RC. 1998. Comparative toxicity of eugenol and its quinone methide metabolite in cultured liver cells using kinetic fluorescence bioassays. Toxicol Appl Pharmacol 149:55-63. PMID:9512727

1997

• Burghardt RC, Barhoumi R. 1997. Analysis of mechanisms of cellular injury on the micrometer scale. Microscopy and Microanalysis 3(2):37-38.
• Meola S, Meola R, Barhoumi R, Miles J, Burghardt RC. 1997. Laser cytometric analysis of permethrin toxicity in insect and mammalian cells. Toxic Subst Mech 16:237-250.
• Wang W, Porter WW, Burghardt RC, Safe SH. 1997. Mechanism of inhibition of MDA-MB-468 cell growth by 2,3,7,8,-tetrachlorodibenzo-p-dioxin. Carcinogenesis 18:925-933. PMID:9163677
• Wang W, Smith, III R, Burghardt RC, Safe SH. 1997. 17 beta-Estradiol-mediated growth inhibition of MDA-MB-468 cells stably transfected with the estrogen receptor: cell cycle effects. Mol Cell Endocrinol 133(1):49-62. PMID:9359472
• Zoran DL, Barhoumi R, Burghardt RC, Chapkin RS, Lupton JR. 1997. Diet and carcinogen alter luminal butyrate concentration and intracellular pH in isolated rat colonocytes. Nutr Cancer 27:222-230. PMID:9101550

1996

• Barhoumi R, Burghardt RC. 1996. Kinetic analysis of the chronology of patulin- and gossypol-induced cytotoxicity in vitro. Fundam Appl Toxicol 30:290-297. PMID:8812278
• Barhoumi R, Mouneimne Y, Phillips TD, Safe SH, Burghardt RC. 1996. Alteration of oxytocin-induced calcium oscillations in Clone 9 cells by toxin-exposure. Fundam Appl Toxicol 33:220-228. PMID:8921340
• Hanneman WH, Legare ME, Barhoumi R, Burghardt RC, Safe SH, Tiffany-Castiglioni E. 1996. Stimulation of calcium uptake in cultured rat hippocampal neurons by 2,3,7,8-tetrachlorodibenxo-p-dioxin (TCDD). Toxicology 112:19-28. PMID:8792845

1995

• Barhoumi R, Bailey RH, Burghardt RC. 1995. Kinetic analysis of glutathione in anchored cells with monochlorobimane. Cytometry Part A 19:226-234. PMID:7736868
• Barhoumi R, Bailey RH, Hutchinson RW, Bowen JA, Burghardt RC. 1995. Enhancement of melphalan toxicity by octanol in ovarian adenocarcinoma cell lines: effects of altered cell-cell communication, glutathione levels and plasma membrane fluidity. Fundam Appl Toxicol 25:70-79. PMID:7601329
• Burghardt RC, Barhoumi R, Sewall TC, Bowen JA. 1995. Cyclic AMP-induces rapid increases in gap junction permeability and changes in the cellular distribution of connexin43. Journal of Membrane Biology 148(3):243-253. doi:10.1007/BF00235042 PMID:8747556
• Hutchinson RW, Barhoumi R, Burghardt RC. 1995. Laser cytometric analysis of gossypol-induced cytotoxicity. Toxic Subst Mech 14:169-184.
• Zhao F, Mayura K, Hutchinson RW, Lewis RP, Burghardt RC, Phillips TD. 1995. Developmental toxicity and structure-activity relationships of chlorophenols using human embryonic palatal mesenchymal cells. Toxicol Lett 78(1):35-42. doi:10.1016/0378-4274(95)99684-A PMID:7604397