Superfund Research Program

Sylvia Daunert

University of Miami
University of Miami
1011 NW 15 St.
Gautier Bldg, Room 116
Miami, Florida 33136
Phone: 305-243-4005
Fax: 305-243-3955
Email: sdaunert@med.miami.edu

Projects

• University of Kentucky: Sensing Superfund Chemicals with Recombinant Systems (1997-2014)

Research Briefs

• 191 - Portable Biosensing Systems
• 113 - Strategies for Quantitative and Rapid Measurements of Arsenic in Water
• 79 - A Cellular Biosensor to Detect Chlorocatechols

Publications

2013

• Choi J, Eum SY, Rampersaud E, Daunert S, Abreu MT, Toborek M. 2013. Exercise attenuates PCB-induced changes in the mouse gut microbiome. Environ Health Perspect 121(6):725-730. doi:10.1289/ehp.1306534 PMID:23632211 PMCID:PMC3672930
• Zhang B, Choi J, Eum SY, Daunert S, Toborek M. 2013. TLR4 signaling is invovled in brain vascular toxicity of PCB153 bound to nanoparticles. PLoS One 8(5):e63159. doi:10.1371/journal.pone.0063159 PMID:23690990 PMCID:PMC3653967

2012

• Geng S, Fang J, Turner KB, Daunert S, Wei Y. 2012. Accumulation and efflux of polychlorinated biphenyls in Escherichia coli. Anal Bioanal Chem 403:2403-2409. doi:10.1007/s00216-012-5835-8 PMID:22349406
• Hamorsky KT, Ensor CM, Dikici E, Pasini P, Bachas LG, Daunert S. 2012. Bioluminescence inhibition assay for the detection of hydroxylated polychlorinated biphenyls. Anal Chem 84(18):7648-7655. doi:10.1021/ac301872u PMID:22908962 PMCID:PMC4127403
• Hamorsky KT, Ensor CM, Pasini P, Daunert S. 2012. A protein switch sensing system for the quantification of sulfate. Anal Biochem 421(1):172-180. doi:10.1016/j.ab.2011.10.023 PMID:22067979 PMCID:PMC3457053
• Knecht LD, Ali N, Wei Y, Hilt JZ, Daunert S. 2012. Nanoparticle-mediated remote control of enzymatic activity. ACS Nano 6(10):9079-9086. doi:10.1021/nn303308v PMID:22989219 PMCID:PMC4127402
• Raut N, O'Connor G, Pasini P, Daunert S. 2012. Engineered cells as biosensing systems in biomedical analysis. Anal Bioanal Chem 402(10):3147-3159. doi:10.1007/s00216-012-5756-6 PMID:22311427

2011

• Hamorsky KT, Dikici E, Ensor CM, Daunert S. 2011. Designer Bioluminescent Systems in Biotechnology. Chapter 13 in: Chemiluminescence and Bioluminescence: Past, Present and Future. Royal Society of Chemistry, Cambridge, United Kingdom.
• Knecht LD, Pasini P, Daunert S. 2011. Bacterial spores as platforms for bioanalytical and biomedical applications. Anal Bioanal Chem 400(4):977-989. doi:10.1007/s00216-011-4835-4 PMID:21380604
• Lewis S, Datta S, Gui M, Coker EL, Huggins F, Daunert S, Bachas LG, Bhattacharyya D. 2011. Reactive nanostructured membranes for water purification. Proc Natl Acad Sci U S A 108(21):8577-8582. doi:10.1073/pnas.1101144108 PMID:21606340 PMCID:PMC3102394
• Sangal A, Pasini P, Daunert S. 2011. Stability of spore-based biosensing systems under extreme conditions. Sens Actuators B Chem 158:377-382. doi:10.1016/j.snb.2011.06.039
• Turner K, Raut N, Pasini P, Daunert S, Michelini E, Cevenini L, Mezzanotte L, Roda A. 2011. Cell-Based Bioluminescent Biosensors. Chapter 15 in: Chemiluminescence and Bioluminescence: Past, Present and Future. Royal Society of Chemistry, Cambridge, United Kingdom. pp.514-545.

2010

• Date A, Pasini P, Daunert S. 2010. Fluorescent and bioluminescent cell-based sensors: strategies for their preservation. Adv Biochem Eng Biotechnol 117:57-75. doi:10.1007/10_2009_22 PMID:20091290
• Date A, Pasini P, Daunert S. 2010. Integration of spore-based genetically engineered whole-cell sensing systems into portable centrifugal microfluidic platforms. Anal Bioanal Chem 398(1):349-356. doi:10.1007/s00216-010-3930-2 PMID:20582692
• Date A, Pasini P, Sangal A, Daunert S. 2010. Packaging sensing cells in spores for long-term preservation of sensors: a tool for biomedical and environmental analysis. Anal Chem 82(14):6098-6103. doi:10.1021/ac1007865 PMID:20560542
• Struss A, Pasini P, Daunert S. 2010. Biosensing Systems Based on Genetically Engineered Whole Cells. Chapter 14 in: Recognition Receptors in Biosensors. Springer, New York, NY. pp.565-598.

2009

• Rowe L, Dikici E, Daunert S. 2009. Engineering bioluminescent proteins: expanding their analytical potential. Anal Chem 81(21):8662-8668. doi:10.1021/ac9007286 PMID:19725502 PMCID:PMC2783804

2006

• Daunert S. 2006. Analytical and bioanalytical chemistry in white waters: 2006 and beyond. Anal Bioanal Chem 384(1):1-3. PMID:16333600
• Feliciano JS, Liu Y, Daunert S. 2006. Novel reporter gene in a fluorescent-based whole cell sensing system. Biotechnol Bioeng 93(5):989-997. PMID:16489629
• Feliciano JS, Pasini P, Deo SK, Daunert S. 2006. Photoproteins as reporters in whole cell sensing systems. Chapter 8 in: Photoproteins in Bioanalysis. Wiley-VCH, New York, NY. pp.131-154.
• Feliciano JS, Xu S, Guan X, Lehmler H, Bachas LG, Daunert S. 2006. ClcR-based biosensing system in the detection of cis-dihydroxylated (chloro-)biphenyls. Anal Bioanal Chem 385(5):807-813. PMID:16791559

2005

• Rothert A, Deo SK, Millner L, Puckett LG, Madou MJ, Daunert S. 2005. Whole-cell-reporter-gene-based biosensing systems on a compact disk microfluidics platform. Anal Biochem 342(1):11-19. PMID:15958175
• Xu S, D'Angelo E, Ghosh D, Feliciano JS, Deo SK, Daunert S. 2005. Detection of polychlorinated biphenyls employing chemical dechlorination followed by biphenyl whole cell sensing system. Toxicol Sci 87(3):287-298. doi:10.1080/02772240400029769

2003

• Feliciano JS, Guan X, Liu Y, D'Angelo E, van der Meer JR, Bachas LG, Daunert S. 2003. Bacterial sensing systems for monitoring superfund chemicals. In: Proceedings of the NAFF (North American Freedom Foundation) Symposium, 2003. Lexington, KY.
• Feliciano JS, Guan X, van der Meer JR, Bachas LG, Daunert S. 2003. Bacterial Biosensing Systems for Monitoring EnvironmentalPollutants:From The Laboratory to the Field. In: Proceedings of the International Symposium on Sensor Science, 2003. Paris, France.
• Feliciano JS, Rothert A, Deo SK, Puckett LG, Millner L, van der Meer JR, Madou MJ, Daunert S. 2003. Bacterial biosensing systems for arsenic detection: from the laboratory to the field. In: Proceedings of the Superfund Basic Research Program Annual Meeting: Integration Perspectives, November 9-12, 2003. Dartmouth College, Hanover, NH.
• Rothert S, Deo SK, Puckett LG, Millner L, Madou MJ, Daunert S. 2003. Adaptation of a whole-cell based reporter gene assay for arsenite and antimonite to a compact disc centrifugal microfluidics patform. In: Proceedings of the 55^th Southeast Regional Meeting of the American Chemical Society (SERMACS), November 16-19, 2003. Atlanta, GA.
• Stocker J, Balluch D, Gsell M, Harms H, Feliciano JS, Daunert S, Malik KA, van der Meer JR. 2003. Development of a set of simple bacterial biosensors for quantitative and rapid measurements of arsenite and arsenate in potable water. Environ Sci Technol 37(20):4743-4750. PMID:14594387
• Xu S, Ghosh A, Feliciano JS, Deo SK, D'Angelo E, Daunert S. 2003. Development of a whole-cell sensing system for PCBs detection. In: Proceedings of the Superfund Basic Research Program Annual Meeting: Integration Perspectives, November 9-12, 2003. Dartmouth College, Hanover, NH.

2002

• Guan X, D'Angelo E, Luo W, Daunert S. 2002. Whole-cell biosensing of 3-chlorocatechol in liquids and soils. Anal Bioanal Chem 374(5):841-847. PMID:12434240

2001

• Butterfield DA, Bhattacharyya D, Daunert S, Bachas LG. 2001. Catalytic biofunctional membranes containing site-specifically immobilized enzyme arrays: a review. J Memb Sci 181(1):29-37.
• Ramanathan S, Shetty RS, Daunert S. 2001. Genetically Engineered Microorganisms as Biosensors for Environmental Toxins. In: Proceedings of the 9^th European Congress on Biotechnology.

2000

• Daunert S, Barrett G, Feliciano JS, Shetty RS, Shrestha S, Smith-Spencer W. 2000. Genetically engineered whole-cell sensing systems: coupling biological recognition with reporter genes. Chem Rev 100(7):2705-2738. PMID:11749302
• Guan X, Ramanathan S, Garris JP, Shetty RS, Ensor CM, Bachas LG, Daunert S. 2000. Chlorocatechol detection based on a clc operon/reporter gene system. Anal Chem 72(11):2423-2427. PMID:10857616

1999

• Ramanathan S, Liu Y, Shetty RS, Guan X, Daunert S. 1999. Luminescence-Based Sensing Systems Employing Genetically Engineered Bacteria. In: Proceedings of the 10^th International Symposium on Bioluminescence and Chemiluminescence. John Wiley & Sons, Chichester, England. pp.601-604.

1998

• Bachas LG, Daunert S, Robertson LW. 1998. Information dissemination and outreach activities at the University of Kentucky. In: Proceedings of the Superfund Basic Research Program (SBRP) Conference, 1998. Boston, MA. pp.69.
• Hernandez EC, Daunert S. 1998. Green fluorescent protein as a novel label in binding assays. Anal Biochem 261(1):113-115.
• Lewis JC, Feltus A, Ensor CM, Ramanathan S, Daunert S. 1998. Applications of reporter genes. Anal Chem 70(17):579A-585A. PMID:9737200
• Ramanathan S, Lewis JC, Kindy MS, Daunert S. 1998. Heterogeneous binding assay for an octapeptide based on a fusion protein with recombinant aequorin. Anal Chim Acta 369:181-188.
• Ramanathan S, Shi W, Rosen BP, Daunert S. 1998. Bacteria-based chemiluminescence sensing system using β-galactosidase under the control of the ArsR regulatory protein of the ars operon. Anal Chim Acta 369:189-195.
• Salins LL, Schauer-Vukasinovic V, Daunert S. 1998. Optical sensing systems based on biomolecular recognition of recombinant proteins. SPIE Proceedings, San Jose, CA 3270:16-24.

1997

• Blair TL, Daunert S, Bachas LG. 1997. Host-Guest Chemistryin Fiber Optic Sensing. In: Biosensors, Current Topics in Biophysics.
• Crofcheck CL, Grosvenor AL, Anderson KW, Lumpp JK, Scott DL, Daunert S. 1997. Detecting biomolecules in pL-vials using the bioluminescence of aequorin. Anal Chem 69(23):4768-4772. PMID:9406527
• Ensor CM, Ramanathan S, Scott DL, Daunert S. 1997. Engineered bacteria can detect toxic metals. Waterworks 3:1-4.
• Feltus A, Ramanathan S, Daunert S. 1997. Cooperative interaction of immobilized avidin with aequorin-biotin conjugates: an aequorin-linked assay for biotin. Anal Biochem 254:62-68.
• Grosvenor AL, Crofcheck CL, Anderson KW, Scott DL, Daunert S. 1997. Calibration of micropipets using the bioluminescent protein aequorin. Anal Chem 69:3115-3118. PMID:9253255
• Lizano S, Ramanathan S, Feltus A, Witkowski A, Daunert S. 1997. Bioluminescence competitive binding assays for biotin based on the photoprotein aequorin. Methods Enzymol 279:296-303. PMID:9211281
• Palet C, Muñoz M, Valiente M, Cynkowski T, Daunert S, Bachas LG. 1997. Molecular recognition of dicarboxylic acids by neutral receptors: selective membrane transport. Anal Chim Acta 343(3):287-294.
• Ramanathan S, Ensor CM, Daunert S. 1997. Metal-resistance bacterial systems in sensors. Trends Biotechnol 15(12):500-506.
• Ramanathan S, Shi W, Rosen BP, Daunert S. 1997. Bioluminescence sensing system for antimonite with subattomole detection based on luciferase reporter genes. Anal Chem 69:3380-3384.
• Schauer-Vukasinovic V, Cullen L, Daunert S. 1997. Rational design of a calcium sensing system based on induced conformational changes of calmodulin. J Am Chem Soc 119:11102-11103.
• Scott DL, Ramanathan S, Shi W, Rosen BP, Daunert S. 1997. Electrochemical-based sensing system for antimonite and arsenite using genetically engineered bacteria. Anal Chem 69:16-20.

1996

• Hentz NG, Daunert S. 1996. Bifunctional fusion proteins of calmodulin and protein A as affinity ligands in protein purification and in the study of protein-protein interactions. Anal Chem 68(22):3939-3944. PMID:8916452
• Hentz NG, Vukasinovic V, Daunert S. 1996. Affinity chromatography for recombinant peptides/proteins based on a calmodulin fusion tail. Anal Chem 68(9):1550-1555. PMID:8815745
• Hentz NG, Vukasinovic V, Diez S, Valiente M, Daunert S. 1996. Affinity Chromatography for Recombinant Proteins. In: Biofunctional Membranes (The Language of Science). Plenum Press, pp.73-81.
• Huang W, Feltus A, Witkowski A, Daunert S. 1996. Homogeneous bioluminescence competitive binding assay for folate based on a coupled glucose-6-phosphate dehydrogenase-bacterial luciferase system. Anal Chem 68(9):1646-1650. PMID:8815749

1995

• Allen JR, Florido A, Young SD, Daunert S, Bachas LG. 1995. Nitrite-selective electrode based on an electropolymerized cobalt phthalocyanine. Electroanalysis 7(8):710-713.
• Daunert S, Bachas LG, Smith-Palmer T. 1995. Gas Sensing Probes. In: Encyclopedia of Analytical Science. Academic Press, pp.4118-4124.
• Diez S, Zhou P, Daunert S, Valiente M. 1995. Immobilized soft-metal affinity system for amino acids based on an 8-hydroxyquinoline-Pd(II) complex: characterization using glycine as a model. Anal Chim Acta 315:339-345.
• Hernandez EC, Witkowski A, Daunert S, Bachas LG. 1995. Potentiometric enzyme electrodes for urea based on electrochemically prepared polypyrrole membranes. Microchimica Acta 121:63-72.
• Witkowski A, Daunert S, Kindy MS, Bachas LG. 1995. Preparation of β-galactosidase conjugates for competitive binding assays by post-translational modification of recombinant proteins. Anal Chem 67:1301-1306.