Superfund Research Program

Wayne L. Backes

LSU Health Sciences Center - New Orleans
1901 Perdido St. P7-1
New Orleans, Louisiana 70112
Phone: 504-568-6557
Email: wbacke@lsuhsc.edu

Projects

• Louisiana State University: Environmentally Persistent Free Radicals Alter Pulmonary Immunologic Homeostasis (2020-2025)
• Louisiana State University: Pollutant-Particle Systems and Xenobiotic Bioactivation (2011-2018)
• Louisiana State University: Pollutant-Particle Systems and Xenobiotic Bioactivation (ARRA Funded) (2009-2011)

Publications

2023

• Connick J, Reed JR, Cawley GF, Saha A, Backes WL. 2023. Functional characterization of CYP1 enzymes: Complex formation, membrane localization and function. J Inorg Biochem 247:112325. doi:10.1016/j.jinorgbio.2023.112325 PMID:37479567
• Reed JR, Guidry JJ, Eyer M, Backes WL. 2023. The influence of lipid microdomain heterogeneity on protein-protein interactions: Proteomic analysis of co-immunoprecipitated binding partners of P450 1A2 and P450 3A in rate liver microsomes. Drug Metab Dispos 51(9):1196-1206. doi:10.1124/dmd.123.001287 PMID:37349115 PMCID:PMC10449098

2022

• Reed JR, Guidry JJ, Backes WL. 2022. Proteomic and bioinformatics analysis of membrane lipid domains after Brij 98 solubilization of uninduced and phenobarbital-induced rat liver microsomes: Defining the membrane localization of the P450 enzyme system. Drug Metab Dispos 50(4):374-385. doi:10.1124/dmd.121.000752 PMID:35094979

2021

• Connick J, Reed JR, Cawley GF, Backes WL. 2021. Heme oxygenase-1 affects cytochrome P450 function through the formation of heteromeric complexes: Interactions between CYP1A2 and heme oxygenase-1. J Biol Chem 296:100030. doi:10.1074/jbc.RA120.015911 PMID:33148696 PMCID:PMC7948974
• Connick J, Reed JR, Cawley GF, Backes WL. 2021. Heteromeric complex formation between human cytochrome P450 CYP1A1 and heme oxygenase-1. Biochem J 478:377-388. doi:10.1042/BCJ20200768 PMID:33394027 PMCID:PMC8552777
• Saha A, Connick J, Reed JR, Lott CS, Backes WL. 2021. Identification of the contact region responsible for the formation of the homomeric CYP1A2-CYP1A2 complex. Biochem J 478(11):2163-2178. doi:10.1042/BCJ20210269 PMID:34032264 PMCID:PMC8522333

2019

• Connick J, Reed JR, Backes WL. 2019. Physical interactions between heme oxygenase 1 and the cytochromes P450 in the endoplasmic reticulum membrane. FASEB J 33:
• Fuchs R, Reed R, Backes WL. 2019. The effect of cholesterol depletion on the localization of CYP2D6. FASEB J 33:
• Saha A, Connick J, Reed JR, Backes WL. 2019. Identifying the regions involved in the interactions between CYP1A2 and CYP2B4. FASEB J 33:

2018

• Connick J, Reed JR, Backes WL. 2018. Characterization of interactions among CYP1A2, CYP2B4, and NADPH-cytochrome P450 reductase: Identifications of specific protein complexes. Drug Metab Dispos 46:197-203. doi:10.1124/dmd.117.078642 PMID:29233819 PMCID:PMC5797933
• Harmon AC, Hebert VY, Cormier SA, Subramanian B, Reed JR, Backes WL, Dugas TR. 2018. Particulate matter containing environmentally persistent free radicals induces AhR-dependent cytokine and reactive oxygen species production in human bronchial epithelial cells. PLoS One 13:18. doi:10.1371/journal.pone.0205412 PMID:30308017 PMCID:PMC6181347

2017

• Pandey AV, Henderson CJ, Ishii Y, Kranendonk M, Backes WL, Zanger UM. 2017. Editorial: Role of protein-protein interactions in metabolism: Genetics, structure, function. Front Pharmacol 8:3-3. doi:10.3389/fphar.2017.00881 PMID:29230176 PMCID:PMC5712015
• Reed JR, Backes WL. 2017. Physical studies of P450-P450 interactions: predicting quaternary structures of P450 complexes in membranes from their X-ray crystal structures. Front Pharmacol 8:28. doi:10.3389/fphar.2017.00028 PMID:28194112 PMCID:PMC5276844

2016

• Brignac-Huber LM, Park J, Reed JR, Backes WL. 2016. Cytochrome P450 organization and function are modulated by endoplasmic reticulum phospholipid heterogeneity. Drug Metab Dispos 44(12):1859-1866. doi:10.1124/dmd.115.068981 PMID:27233287 PMCID:PMC5118634
• Reed JR, Backes WL. 2016. The functional effects of physical interactions involving cytochromes P450: putative mechanisms of action and the extent of these effects in biological membranes. J Mol Biol 48(3):453-469. doi:10.1080/03602532.2016.1221961 PMID:27500687 PMCID:PMC5108515
• Scott EE, Wolf CR, Otyepka M, Humphreys SC, Reed JR, Henderson CJ, McLaughlin LA, Paloncyova M, Navratilova V, Berka K, Anzenbacher P, Dahal UP, Barnaba C, Brozik JA, Jones JP, Estrada DF, Laurence JS, Park J, Backes WL. 2016. The role of protein-protein and protein-membrane interactions on P450 function. Drug Metab Dispos 44(4):576-590. doi:10.1124/dmd.115.068569 PMID:26851242 PMCID:PMC4810767

2015

• Park J, Reed JR, Backes WL. 2015. The localization of cytochrome P450s CYP1A1 and CYP1A2 into different lipid microdomains is governed by their N-terminal and internal protein regions. J Biol Chem 290(49):29449-29460. doi:10.1074/jbc.M115.687103 PMID:26468279 PMCID:PMC4705947
• Reed JR, dela Cruz AL, Lomnicki S, Backes WL. 2015. Environmentally persistent free radical-containing particulate matter competitively inhibits metabolism by cytochrome P450 1A2. Toxicol Appl Pharmacol 289(2):223-230. doi:10.1016/j.taap.2015.09.021 PMID:26423927 PMCID:PMC4651829
• Reed JR, dela Cruz AL, Lomnicki S, Backes WL. 2015. Inhibition of cytochrome P450 2B4 by environmentally persistent free radical-containing particulate matter. Biochem Pharmacol 95(2):126-132. doi:10.1016/j.bcp.2015.03.012 PMID:25817938 PMCID:PMC4406862

2014

• Johnson EF, Connick J, Reed JR, Backes WL, Desai MC, Xu L, Estrada F, Laurence JS, Scott EE. 2014. Correlating structure and function of drug-metabolizing enzymes: progress and ongoing challenges. Drug Metab Dispos 42(1):9-22. doi:10.1124/dmd.113.054627 PMID:24130370 PMCID:PMC3876788
• Reed JR, Brignac-Huber LM, Backes WL, Park J. 2014. Cytochrome P450 system proteins reside in different regions of the endoplasmic reticulum. Biochem J 464(2):241-249. doi:10.1042/BJ20140787 PMID:25236845 PMCID:PMC4314108
• Reed JR, Cawley GF, Ardoin TG, Dellinger B, Lomnicki S, Kiruri LW, Backes WL. 2014. Environmentally persistent free radicals inhibit cytochrome P450 activity in rat liver microsomes. Toxicol Appl Pharmacol 277(2):200-209. doi:10.1016/j.taap.2014.03.021 PMID:24713513 PMCID:PMC4049543

2013

• Park J, Reed JR, Brignac-Huber LM, Cawley GF, Eyer M, Backes WL. 2013. Organization of cytochrome P450 system components in the endoplasmic reticulum. FASEB J 892.3:
• Reed JR, Cawley GF, Backes WL. 2013. Interactions between cytochromes P450 2B4 (CYP2B4) and 1A2 (CYP1A2) lead to alterations in toluene disposition and P450 uncoupling. Biochemistry 52(23):4003-4013. doi:10.1021/bi400422a PMID:23675771 PMCID:PMC3750074

2012

• Reed JR, Backes WL. 2012. Formation of P450 · P450 complexes and their effect on P450 function. Pharmacol Ther 133:299-310. doi:10.1016/j.pharmthera.2011.11.009 PMID:22155419 PMCID:PMC3272114